Anticorps Polyclonal de lapin anti-RRM1

• Phare
• Validé par KD/KO

RRM1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

49

N° de cat : 10526-1-AP

Synonymes

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1

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Galerie de données de validation

• 

  WB analysis of HeLa using 10526-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10526-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10526-1-AP

  A549 cells (shcontrol and shRNA of RRM1) were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000. .

• 

  WB analysis of BxPC-3 using 10526-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10526-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 10526-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10526-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 10526-1-AP

  IP result of anti-RRM1 (IP:10526-1-AP, 5ug; Detection:10526-1-AP 1:800) with K-562 cells lysate 8000ug.

• 

  IHC staining of human breast cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10526-1-AP (RRM1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10526-1-AP (RRM1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10526-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of Raji using 10526-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules BxPC-3, cellules HEK-293, cellules HeLa, cellules K-562, cellules U-251
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 33 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10526-1-AP cible RRM1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain, souris, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRM1 Protéine recombinante Ag0789
• Nom complet: ribonucleotide reductase M1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC006498
• Symbole du gène: RRM1
• Identification du gène (NCBI): 6240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide 

synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair.

What is the molecular weight of RRM1?

The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed 

of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2 

subunits in non-dividing quiescent cells (PMID: 17416930).

What is the subcellular localization of RRM1?

RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2. 

However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953).

What is the tissue expression pattern of RRM1?

RRM1 is ubiquitously expressed.

How is RRM1 expression regulated during the cell cycle and upon DNA damage?

Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase. 

However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to 

RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins 

or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592).

Publications

Species	Application	Title
human	WB, IF	N Engl J Med; DNA synthesis and repair genes RRM1 and ERCC1 in lung cancer.; Authors - Zhong Zheng
human	IF	Lancet Oncol; The ribonucleotide reductase large subunit (RRM1) as a predictive factor in patients with cancer.; Authors - Lars Petter Jordheim
human	WB	Clin Cancer Res; PMID: 25224279; Authors - Martin Kerr ; KD Validated
human	WB, IHC, IP	Clin Cancer Res; PMID: 26228206; Authors - Francesca Vena
human	IHC	Ann Oncol; PMID: 23038758; Authors - Vilmar A C AC
	IHC	Oncotarget; Impact of molecular profiling on overall survival of patients with advanced ovarian cancer.; Authors - Thomas J Herzog

Produits associés

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11661-1-AP

ERCC1 Polyclonal antibody

14586-1-AP

P53 Polyclonal antibody

10442-1-AP

ERCC2 Polyclonal antibody

10818-1-AP

TPTE Polyclonal antibody

12982-1-AP