ERCC2 Polyclonal antibody

ERCC2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10818-1-AP

Synonyms

BTF2 p80, COFS2, CXPD, EM9, ERCC2, TFIIH 80 kDa subunit, TFIIH p80, TTD, XPD, XPDC



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HEK-293 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10818-1-AP targets ERCC2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERCC2 fusion protein Ag1038
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 2
Calculated molecular weight 87 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC008346
Gene symbol ERCC2
Gene ID (NCBI) 2068
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.

Protocols

Product Specific Protocols
WB protocol for ERCC2 antibody 10818-1-APDownload protocol
IHC protocol for ERCC2 antibody 10818-1-APDownload protocol
IP protocol for ERCC2 antibody 10818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

PMID: 27449101

Authors - Henan Zhao
humanIHC

Cancer Sci

Very low prevalence of XPD K751Q polymorphism and its association with XPD expression and outcomes of FOLFOX-4 treatment in Asian patients with colorectal carcinoma.

Authors - Lai Jiun-I JI
humanIHC

Methods

Synchronous detection of miRNAs, their targets and downstream proteins in transferred FFPE sections: applications in clinical and basic research.

Authors - Zhao Jin-yao JY
humanIHC

Am J Cancer Res

Predictive value of ERCC1, ERCC2, ERCC4, and glutathione S-Transferase Pi expression for the efficacy and safety of FOLFIRINOX in patients with unresectable pancreatic cancer.

Authors - Shun Tezuka

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zizhao (Verified Customer) (08-15-2018)

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Primary Antibody Dilution: 1: 1000
  • Cell Tissue Type: human keratinocytes