Anticorps Polyclonal de lapin anti-S100B

S100B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(136)

N° de cat : 15146-1-AP

Synonymes

S100 beta, Protein S100 B, Protein S100-B, S100 calcium-binding protein B, S-100 protein beta chain



Applications testées

Résultats positifs en WBtissu cérébral de souris, cellules A375, cellules C6, cellules U-251, tissu cérébral de rat, tissu testiculaire humain
Résultats positifs en IHCtissu de mélanome malin humain, tissu cérébral de rat, tissu cérébral de souris, tissu d'appendicite humain, tissu de gliome humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de souris, tissu de mélanome malin humain
Résultats positifs en IF/ICCastrocytes humains, cellules A375
Résultats positifs en FC (Intra)cellules A375

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:20000
Immunohistochimie (IHC)IHC : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15146-1-AP cible S100B dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène S100B Protéine recombinante Ag7440
Nom complet S100 calcium binding protein B
Masse moléculaire calculée 11 kDa
Poids moléculaire observé 11 kDa
Numéro d’acquisition GenBankBC001766
Symbole du gène S100 Beta
Identification du gène (NCBI) 6285
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100B belongs to the EF-hand calcium binding proteins and is found primarily in astrocytes in the central nervous system (CNS). S100B has a variety of functions, including calcium homeostasis, cell proliferation, differentiation, migration, and survival, as well as neurite outgrowth and regeneration.

 1. What is the molecular weight of S100B?

S100B protein is composed of non-covalently linked homodimers of 11 kDa size.

2. What is the subcellular localization of S100B?

S100B localizes to the nucleus and cytoplasm, associating with intracellular membranes, the centrosomes, microtubules, and type III intermediate filaments (PMID: 19110011). Additionally, it can be released from astrocytes into the extracellular space and can enter the bloodstream.

3. What is the expression pattern of S100B?

S100B is predominantly expressed in astrocytes and maturing oligodendrocytes but is also present in other cell types such as kidney epithelial cells, neural progenitor cells, pituicytes, ependymocytes, chondrocytes, adipocytes, melanocytes, Langerhans cells, dendritic cells, certain lymphocyte subpopulations, skeletal myofibers, myoblasts, and muscle satellite cells (PMID: 19110011). S100B is a commonly used marker of Schwann cells and reactive astrocytes in ICC, IHC, and WB applications.

4. What is the diagnostic use of S100B in the clinic?

S100B is naturally secreted by astrocytes into the extracellular space and low amounts of S100B can pass through the brain-blood barrier and enter the bloodstream. Elevated levels of S100B in the serum are observed in patients with traumatic head injuries, as well as in patients suffering from neurodegenerative diseases (PMID: 30144068). This increase in S100B levels is attributed to the elevated secretion of S100B protein from astrocytes as part of the physiological response to the injury, as well as to the physical damage of astrocytes and increased blood-brain barrier permeability.


Protocole

Product Specific Protocols
WB protocol for S100B antibody 15146-1-APDownload protocol
IHC protocol for S100B antibody 15146-1-APDownload protocol
IF protocol for S100B antibody 15146-1-APDownload protocol
FC protocol for S100B antibody 15146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian
mouseIHC

Nature

Regulation of intestinal immunity and tissue repair by enteric glia

Authors - Fränze Progatzky
mouseIHC

Cell

Mechanoreceptor synapses in the brainstem shape the central representation of touch.

Authors - Brendan P Lehnert
humanIF

Sci Bull (Beijing)

Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes

Authors - Jiangbing Xiang
mouseIF

Nat Commun

Alk1 acts in non-endothelial VE-cadherin+ perineurial cells to maintain nerve branching during hair homeostasis

Authors - Gopal Chovatiya
mouseIF

Neuron

γ-Protocadherins control synapse formation and peripheral branching of touch sensory neurons

Authors - Shan Meltzer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Clarisse (Verified Customer) (08-08-2022)

This antibody works very well as an astrocyte marker. In the image: in green mouse anti-S100B (1:500 - 66616-1-Ig) and in magenta rabbit anti-S100B (1:500 - 15146-1-AP). The rabbit antibody has a better performance.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human cerebral organoids
S100B Antibody Immunofluorescence validation (1:500 dilution) in Human cerebral organoids (Cat no:15146-1-AP)
FH

Tongcheng (Verified Customer) (09-08-2021)

This antibody works perfect on human astrocytes.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: astrocyte
FH

Sonia (Verified Customer) (10-21-2019)

Works really well on mouse sciatic nerve cross section (cryostat)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/400
  • Cell Tissue Type: mouse scitic nerve
S100B Antibody Immunofluorescence, validation (1/400 dilution) in mouse scitic nerve (Cat no:15146-1-AP)
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