Anticorps Polyclonal de lapin anti-SCAMP2

SCAMP2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 15119-1-AP

Synonymes

Secretory carrier-associated membrane protein 2

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 15119-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15119-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15119-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15119-1-AP

  IP result of anti-SCAMP2 (IP:15119-1-AP, 4ug; Detection:15119-1-AP 1:500) with HepG2 cells lysate 3300 ug.

• 

  IHC staining of human pancreas cancer using 15119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15119-1-AP (SCAMP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 15119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15119-1-AP (SCAMP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HEK-293 using 15119-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SCAMP2 antibody (15119-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 15119-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug SCAMP2 Polyclonal antibody (15119-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu hépatique humain, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15119-1-AP cible SCAMP2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCAMP2 Protéine recombinante Ag7190
• Nom complet: secretory carrier membrane protein 2
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC001376
• Symbole du gène: SCAMP2
• Identification du gène (NCBI): 10066
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCAMP2 functions in post-Golgi recycling pathways. It acts as a recycling carrier to the cell surface. It couples Arf6-stimulated PLD activity to exocytosis and links this process to formation of fusion pores. SCAMP2 is marker for secretory vesicles and tobacco BY-2 cells as a model system. The MW of SCAMP2 is 36-39 kDa with modification.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.; Authors - Feiyu Mao

{{ptg:RelatedPrimaryAntibodies}}