Anticorps Polyclonal de lapin anti-Surfactant Protein A

Surfactant Protein A Polyclonal Antibody for FC, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11850-1-AP

Synonymes

Alveolar proteinosis protein, COLEC4, Collectin 4, PSAP, PSP A, PSPA, SFTP1, SFTPA, SFTPA1, SFTPA1B, SFTPA2, SP A, SP A1, Surfactant Protein A, surfactant protein A1

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Galerie de données de validation

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  IHC staining of human lung using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of A549 using 11850-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Surfactant Protein A (11850-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu pulmonaire humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu de cancer du poumon humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules A549,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

11850-1-AP cible Surfactant Protein A dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Surfactant Protein A Protéine recombinante Ag2417
• Nom complet: surfactant protein A1
• Masse moléculaire calculée: 248 aa, 26 kDa
• Numéro d’acquisition GenBank: BC026229
• Symbole du gène: SFTPA1
• Identification du gène (NCBI): 653509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Surfactant protein A (SP-A), is the major protein component of pulmonary surfactant involved in surfactant-related function or structure and in the regulation of inflammatory processes and innate host defens. In humans and primates, SP-A1 (SFTPA1) and SP-A2 (SFTPA2) genes encode SP-A, and these two SP-A genes have high homology (PMID: 34484180, PMID: 19392648). This antibody can recognize both SP-A1 (SFTPA1) and SP-A2 (SFTPA2).

Publications

Species	Application	Title
pig	WB	Sci Total Environ; Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets; Authors - Xiaotong Wang
mouse	WB	Int J Mol Sci; Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.; Authors - Guangying Yang
human,mouse	IHC	Cancer Immunol Immunother; SFTPA1 is a potential prognostic biomarker correlated with immune cell infiltration and response to immunotherapy in lung adenocarcinoma.; Authors - Lu Yuan
mouse	WB	Biochem Biophys Res Commun; Increased mortality from influenza infection in long-chain acyl-CoA dehydrogenase knockout mice.; Authors - Apurva Shinde
human	WB	Environ Toxicol; Silica nanoparticle exposure inhibits surfactant protein A and B in A549 cells through ROS-mediated JNK/c-Jun signaling pathway.; Authors - Xiaojing Yang
mouse	WB	J Thorac Dis; Deletion of LCMR1 in alveolar type II cells induces lethal impairment of lung structure and function in adult mice; Authors - Yueming Wang

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C