Anticorps Monoclonal anti-SOD2

• Phare
• Validé par KD/KO

SOD2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

35

CloneNo.

3A6C2

Avis (1)

N° de cat : 66474-1-Ig

Synonymes

IPO B, Mn SOD, MNSOD, SOD2, SODM

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Galerie de données de validation

• 

  WB analysis using 66474-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66474-1-Ig (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HeLa using 66474-1-Ig

  WB result of SOD2 antibody (66474-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD2 transfected HeLa cells.

• 

  WB analysis using 66474-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66474-1-Ig (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66474-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66474-1-Ig (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human liver using 66474-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:300 (under 40x lens).

• 

  IF Staining of HUVEC using 66474-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (66474-1-Ig, Clone: 3A6C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66474-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SOD2 (66474-1-Ig, Clone:3A6C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HeLa, cellules RAW 264.7, cellules ROS1728, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HUVEC,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 30 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

66474-1-Ig cible SOD2 dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SOD2 Protéine recombinante Ag21388
• Nom complet: superoxide dismutase 2, mitochondrial
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC016934
• Symbole du gène: SOD2
• Identification du gène (NCBI): 6648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Publications

Species	Application	Title
mouse	WB,IF	J Control Release; A cerium oxide loaded glycol chitosan nano-system for the treatment of dry eye disease.; Authors - Fan Yu
mouse	WB	Stem Cell Res Ther; Apoptotic vesicles resist oxidative damage in noise-induced hearing loss through activation of FOXO3a-SOD2 pathway; Authors - Xiaotong Huang
human	WB	Oxid Med Cell Longev; HO-1 Contributes to Luteolin-Triggered Ferroptosis in Clear Cell Renal Cell Carcinoma via Increasing the Labile Iron Pool and Promoting Lipid Peroxidation.; Authors - Shangting Han
mouse	WB	Mol Ther Nucleic Acids; Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis; Authors - Ni Zeng
pig	WB	J Anim Sci Biotechnol; Resveratrol and its derivative pterostilbene ameliorate intestine injury in intrauterine growth-retarded weanling piglets by modulating redox status and gut microbiota.; Authors - Yanan Chen
human	WB	J Anim Sci Biotechnol; Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets.; Authors - Hao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (07-30-2020)

This antibody works very well at 1:2000 dilution with O/N incubation.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Fallopian tube epithelial cells

SOD2 Antibody Western Blot, validation (1:2000 dilution) in Fallopian tube epithelial cells (Cat no:66474-1-Ig)