Anticorps Polyclonal de lapin anti-SUFU

• Phare
• Validé par KD/KO

SUFU Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 26759-1-AP

Synonymes

PRO1280, SUFUH, Suppressor of fused homolog, UNQ650/PRO1280

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Galerie de données de validation

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  WB analysis using 26759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26759-1-AP (SUFU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26759-1-AP

  WB result of SUFU antibody (26759-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUFU transfected HeLa cells.

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  WB analysis of C6 using 26759-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 26759-1-AP (SUFU Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26759-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26759-1-AP (SUFU Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 26759-1-AP

  IP result of anti-SUFU (IP:26759-1-AP, 4ug; Detection:26759-1-AP 1:1000) with NIH/3T3 cells lysate 1600 ug.

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  IHC staining of human kidney using 26759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26759-1-AP (SUFU Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26759-1-AP (SUFU Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26759-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SUFU antibody (26759-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 26759-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug SUFU Polyclonal antibody (26759-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293, cellules NIH/3T3
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

26759-1-AP cible SUFU dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUFU Protéine recombinante Ag25200
• Nom complet: suppressor of fused homolog (Drosophila)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC013291
• Symbole du gène: SUFU
• Identification du gène (NCBI): 51684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sufu (Suppressor of fused) is a central regulator of Hh (Hedgehog) signaling and acts as a tumor suppressor by maintaining the Gli (Glioma-associated oncogene homolog) transcription factors inactive. Loss of Sufu function promotes tumorigenesis.

Publications

Species	Application	Title
mouse	WB	Theranostics; Genetic and pharmacological activation of Hedgehog signaling inhibits osteoclastogenesis and attenuates titanium particle-induced osteolysis partly through suppressing the JNK/c-Fos-NFATc1 cascade.; Authors - Liwei Zhang
mouse	WB	Phytother Res; Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway; Authors - Cheng-Hui He
human	WB	Phytother Res; Ursolic acid inhibits the activation of smoothened-independent non-canonical hedgehog pathway in colorectal cancer by suppressing AKT signaling cascade.; Authors - Li Chen
mouse	IHC	Sci Rep; Suppression of Hedgehog signaling is required for cementum apposition.; Authors - Hwajung Choi
human	WB	Comput Intell Neurosci; Inhibitory Effect of Ursolic Acid on Proliferation and Migration of Renal Carcinoma Cells and Its Mechanism.; Authors - Xiao Lyu
human	IF	Cell Rep; The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling.; Authors - Leah Schembs

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-15-2024)

I tried immunofluorescence in NIH-3T3 and in IMCD3 cells. I did PFA 4% followed by cold methanol 5min. Then blocking and ab solution in BSA 1%. Surprisingly I obtained some signal and it even overlap with the ARL13B signal! But when I used SuFuKO cells, I still have this signal so I'm not convinced by this antibody. The picture is done in starvation (no ShhN) nuclei-SuFu-ARL13b-Merged

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/250
• Cell Tissue Type: NIH-3T3

SUFU Antibody Immunofluorescence validation (1/250 dilution) in NIH-3T3 (Cat no:26759-1-AP)

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