Anticorps Monoclonal anti-VDAC1/Porin

VDAC1/Porin Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(40)

CloneNo.

1E2C7

N° de cat : 66345-1-Ig

Synonymes

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis using 66345-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IF Staining of mouse liver using 66345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using VDAC1/Porin antibody (66345-1-Ig, Clone: 1E2C7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 66345-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug VDAC1/Porin Monoclonal antibody (66345-1-Ig, Clone:1E2C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG3 isotype control Mouse McAb (66360-4-Ig, Clone: 1H4A5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, cellules RAW264.7
• Résultats positifs en IF-P: tissu hépatique de souris,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 30 publications below
• IHC: See 2 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66345-1-Ig cible VDAC1/Porin dans les applications de WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: voltage-dependent anion channel 1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: NM_003374
• Symbole du gène: Porin
• Identification du gène (NCBI): 7416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma; Authors - Qifan Hu
	WB	Proc Natl Acad Sci U S A; Ferroptosis as a target for protection against cardiomyopathy.; Authors - Xuexian Fang
human	WB	Free Radic Biol Med; The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.; Authors - Zhiguo Li
mouse		Diabetes; PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.; Authors - Chenrui Li
mouse	WB	Oxid Med Cell Longev; The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury.; Authors - Wei Tang
mouse	WB	Cell Death Discov; Idebenone improves motor dysfunction, learning and memory by regulating mitophagy in MPTP-treated mice.; Authors - Junqiang Yan

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