Anticorps Polyclonal de lapin anti-Vimentin

• Phare
• Validé par KD/KO

Vimentin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

1390

Avis (10)

N° de cat : 10366-1-AP

Synonymes

VIM, vimentin

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Galerie de données de validation

• 

  WB analysis using 10366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10366-1-AP (Vimentin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat cells using 10366-1-AP

  WB result of Vimentin antibody (10366-1-AP, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

• 

  WB analysis of NIH/3T3 using 10366-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10366-1-AP (VIM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10366-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10366-1-AP (VIM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10366-1-AP (VIM antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human breast cancer using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10366-1-AP (Vimentin antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10366-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10366-1-AP (Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10366-1-AP

  Immunofluorescent analysis of HepG2 cells, using VIM antibody 10366-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 10366-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Vimentin antibody (10366-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'amygdalite humain, tissu de cancer du sein humain, tissu de cirrhose hépatique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, tissu de cancer du sein humain, tissu rénal humain
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 1166 publications below
• IHC: See 217 publications below
• IF: See 281 publications below
• IP: See 8 publications below
• ELISA: See 1 publications below
• FC: See 2 publications below
• CoIP: See 5 publications below

Informations sur le produit

10366-1-AP cible Vimentin dans les applications de WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Vimentin Protéine recombinante Ag0489
• Nom complet: vimentin
• Masse moléculaire calculée: 466 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC000163
• Symbole du gène: VIM
• Identification du gène (NCBI): 7431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Publications

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
mouse	IF	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
human		Adv Sci (Weinh); PMID: 31728273; Authors - Qingming Tang
	IHC	Circ Res; Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis.; Authors - Jiangbin Wu
mouse	IF	Sci Adv; YAP drives fate conversion and chemoresistance of small cell lung cancer.; Authors - Qingzhe Wu
human	WB	Nat Commun; SCFFBW7-mediated degradation of Brg1 suppresses gastric cancer metastasis.; Authors - Li-Yu Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vernon (Verified Customer) (01-29-2024)

Western blot signal is great with 30 sec exposure secondary antibody 1:5000 1hr. at room temp

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Ewing sarcoma cell line

Vimentin Antibody Western Blot validation (1:2000 dilution) in Ewing sarcoma cell line (Cat no:10366-1-AP)

FH

Tobias (Verified Customer) (06-16-2023)

Works well in IF

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200

FH

Emma (Verified Customer) (02-22-2022)

Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

FH

Yuki (Verified Customer) (01-07-2022)

Consistently works antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: gut duct tissue

Vimentin Antibody Immunofluorescence validation (1:150 dilution) in gut duct tissue (Cat no:10366-1-AP)

FH

Hala (Verified Customer) (07-08-2021)

Works veryyyy gooddd

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000 - 1/2000
• Cell Tissue Type: hepG2 transduced with TDO2 gene, liver cancer cells

FH

Hala (Verified Customer) (04-11-2021)

very good antibody, works very well

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2 transduced with TDO2 gene

Vimentin Antibody Western Blot validation (1/1000 dilution) in hepG2 transduced with TDO2 gene (Cat no:10366-1-AP)

FH

wei (Verified Customer) (04-27-2020)

I tried in C2C12 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

Vimentin Antibody Western Blot validation (1:1000 dilution) in C2C12 (Cat no:10366-1-AP)

FH

Patricia (Verified Customer) (02-25-2020)

Works well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer

FH

Kishor (Verified Customer) (01-30-2019)

Good results in western blotting but I could not see consistent results in IF

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

FH

Jaime (Verified Customer) (01-24-2018)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Mouse ventricle frozen sections

Vimentin Antibody Immunofluorescence validation (1/200 dilution) in Mouse ventricle frozen sections (Cat no:10366-1-AP)

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