Anticorps Polyclonal de lapin anti-XBP1S/XBP1U

• Phare
• Validé par KD/KO

XBP1S/XBP1U Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, FC (Intra), ChIP, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (1)

N° de cat : 24168-1-AP

Synonymes

XBP1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1

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Galerie de données de validation

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  WB analysis of HEK-293 using 24168-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24168-1-AP

  WB result of XBP1S/XBP1U antibody (24168-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-XBP1S/XBP1U transfected HepG2 cells.

• 

  WB analysis using 24168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1S/XBP1U antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24168-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1S/XBP1U antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 24168-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XBP1S/XBP1U (24168-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules Jurkat, cellules Raji
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

24168-1-AP cible XBP1S/XBP1U dans les applications de WB, IHC, FC (Intra), ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XBP1S/XBP1U Protéine recombinante Ag21454
• Nom complet: X-box binding protein 1
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: ~32 kDa; ~60 kDa
• Numéro d’acquisition GenBank: BC000938
• Symbole du gène: XBP1
• Identification du gène (NCBI): 7494
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.

Publications

Species	Application	Title
rat	WB,IHC	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou
human	WB	J Ethnopharmacol; Network pharmacology, molecular docking, and in vitro experimental verification of the mechanism of Guanxining in treating diabetic atherosclerosis; Authors - Wenting Wang
mouse	WB	Bioorg Chem; Dihydroartemisinin alleviates steatosis and inflammation in nonalcoholic steatohepatitis by decreasing endoplasmic reticulum stress and oxidative stress.; Authors - Tongxi Li
human	WB	J Virol; Inositol requiring enzyme 1α promotes Zika virus infection through regulating stearoyl-CoA desaturase 1 mediated lipid metabolism.; Authors - Yanxia Huang ; KD Validated
mouse	WB	Exp Ther Med; Apelin‑13 reduces high glucose‑induced mitochondrial dysfunction in cochlear hair cells by inhibiting endoplasmic reticulum stress; Authors - Zhiqiang Huo
mouse	WB	J Zhejiang Univ Sci B; Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure; Authors - Xue YANG

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

• Applications: Other,

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