Anticorps Recombinant de lapin anti-Alpha smooth muscle actin specific

• Phare
• Validé par KD/KO

Alpha smooth muscle actin specific Recombinant Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, porc, poulet, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(37)

CloneNo.

5H7

N° de cat : 80008-1-RR

Synonymes

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 5H7

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Galerie de données de validation

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  WB analysis using 80008-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis using 80008-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80008-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 80008-1-RR

  WB result of smooth muscle actin specific antibody (80008-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin specific transfected A549 cells.

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  WB analysis using 80008-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80008-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 80008-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 80008-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using Alpha smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of mouse embryo using 80008-1-RR

  E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

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  FC experiment of C2C12 using 80008-1-RR

  1x10^6 C2C12 cells were intracellularly stained with 0.5 ug smooth muscle actin specific Recombinant antibody (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, cellules A549, côlon de porc, estomac de porc, estomac de rat, tissu de côlon de rat, tissu de jabot de poulet, tissu de poisson-zèbre, tissu veineux de porc
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain, tissu d'appendicite humain, tissu de côlon de rat, tissu de côlon de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris, tissu embryonnaire de souris
• Résultats positifs en IF-Fro: tissu d'intestin grêle de souris,
• Résultats positifs en FC (Intra): cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 26 publications below
• IHC: See 12 publications below
• IF: See 15 publications below

Informations sur le produit

80008-1-RR cible Alpha smooth muscle actin specific dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, porc, poulet, rat, souris

• Réactivité: Humain, poisson-zèbre, porc, poulet, rat, souris
• Réactivité citée: rat, Humain, porc, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: actin, alpha 2, smooth muscle, aorta
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_001613
• Symbole du gène: Alpha smooth muscle actin
• Identification du gène (NCBI): 59
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Publications

Species	Application	Title
human	WB,IF	Gastroenterology; Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells; Authors - Decai Wang
mouse	WB,IF	Biomaterials; Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure; Authors - Lingjun Tong
human	IF	JCI Insight; Targeting hyaluronan synthesis enhances the therapeutic effectiveness of biologics in inflammatory bowel disease; Authors - Peng Xiao
rat	WB	Phytomedicine; Quercetin improves airway remodeling in COPD rats by suppressing phenotypic switch of ASMCs via inhibiting the Wnt5a/β-catenin pathway; Authors - Hui Zhou
mouse	WB,IHC	Pharmacol Res; MicroRNA-24-3p alleviates cardiac fibrosis by suppressing cardiac fibroblasts mitophagy via downregulating PHB2.; Authors - Yue Zhang
mouse,human	WB,IHC,IF	Int Immunopharmacol; TSLP/TSLPR promotes renal fibrosis by activating STAT3 in renal fibroblasts; Authors - Decai Wang

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