Anticorps Monoclonal anti-Beta Actin

Beta Actin Monoclonal Antibody for FC (Intra), IF, WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, poisson-zèbre, rat, singe, souris, Hamster

Applications

WB, IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications

4

CloneNo.

2D4H5

N° de cat : CL488-66009

Synonymes

ACTB, actin, actin, beta, Beta Actin

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Galerie de données de validation

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  WB analysis using CL488-66009

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66009 (Beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using CL488-66009

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Beta Actin antibody (CL488-66009, Clone: 2D4H5 ) at dilution of 1:200.

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  IF Staining of U2OS using CL488-66009

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 Beta Actin antibody (CL488-66009, Clone: 2D4H5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-66009

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Beta Actin (CL488-66009, Clone:2D4H5) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa
• Résultats positifs en IF: cellules MDCK, cellules U2OS
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

CL488-66009 cible Beta Actin dans les applications de WB, IF, FC (Intra) et montre une réactivité avec des échantillons canin, Humain, poisson-zèbre, rat, singe, souris, Hamster

• Réactivité: canin, Humain, poisson-zèbre, rat, singe, souris, Hamster
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: actin, beta
• Masse moléculaire calculée: 42 kDa
• Numéro d’acquisition GenBank: NM_001101
• Symbole du gène: ACTB
• Identification du gène (NCBI): 60
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL488 (Ex/Em 488 nm/515 nm) conjugated.

Publications

Species	Application	Title
rat	WB	Br J Pharmacol; Fingolimod ameliorates schizophrenia-like cognitive impairments induced by phencyclidine in male rats; Authors - Xueli Yu
human	WB	Mediators Inflamm; Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2; Authors - Junqi Fu
human	WB	Oncol Lett; CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process; Authors - Yang Zhang
mouse	IF	Nanoscale; Effects of hydrophilic fullerene nanoarchitectured structures on the behaviour of neural stem cells.; Authors - Chui-Wei Wong

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C