Anticorps Monoclonal anti-c-SRC

c-SRC Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris, Hamster

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

CloneNo.

5E10C4

N° de cat : CL594-60315

Synonymes

SRC, 5E10C4, c SRC, cSRC, EC:2.7.10.2

---

Galerie de données de validation

• 

  IF Staining of human breast cancer using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

• 

  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL594-60315 (c-SRC antibody) at dilution of 1:100.

• 

  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

• 

  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

• 

  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

• 

  FC experiment of MCF-7 using CL594-60315

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human c-SRC (CL594-60315, Clone:5E10C4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-60315 cible c-SRC dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris, Hamster

• Réactivité: Humain, souris, Hamster
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: c-SRC Protéine recombinante Ag22306
• Nom complet: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC011566
• Symbole du gène: SRC
• Identification du gène (NCBI): 6714
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Publications

Species	Application	Title
human	IF	Mol Cancer; Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.; Authors - Zhenzhen Pan

{{ptg:RelatedPrimaryAntibodies}}