Anticorps Monoclonal anti-Alpha smooth muscle actin specific

Alpha smooth muscle actin specific Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications(4)

CloneNo.

1E9A11

N° de cat : CL647-67735

Synonymes

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form

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Galerie de données de validation

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  IF Staining of mouse heart using CL647-67735

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11 ) at dilution of 1:100, CoraLite®488 Cardiac Troponin I antibody (CL488-66376, Clone: 1D5D6, green), CoraLite®594 N-cadherin antibody (CL594-22018, red). DAPI (blue).

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  IF Staining of mouse heart using CL647-67735

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11 ) at dilution of 1:200.

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  FC experiment of C2C12 using CL647-67735

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human smooth muscle actin specific (CL647-67735, Clone:1E9A11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en FC (Intra): cellules C2C12,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

CL647-67735 cible Alpha smooth muscle actin specific dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: actin, alpha 2, smooth muscle, aorta
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_001613
• Symbole du gène: Alpha smooth muscle actin
• Identification du gène (NCBI): 59
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Publications

Species	Application	Title
human	IF	J Med Virol; Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis; Authors - Arun Das
human	IF	Lab Invest; HSPB6 deficiency promotes the development of aortic dissection and rupture; Authors - Shiqi Gao
human		Adv Sci (Weinh); Patient-Derived Tumor Organoids Combined with Function-Associated ScRNA-Seq for Dissecting the Local Immune Response of Lung Cancer; Authors - Chang Liu
mouse	FC	Nat Commun; FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination; Authors - Chuxiang Lei

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