Anticorps Polyclonal de lapin anti-RPS27A

RPS27A Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14946-1-AP

Synonymes

CEP80, HUBCEP80, ribosomal protein S27a, RPS27A, UBA80, UBCEP1, UBCEP80, ubiquitin

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 14946-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14946-1-AP (RPS27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14946-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14946-1-AP (RPS27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14946-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS27A antibody (14946-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14946-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RPS27A (14946-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu de cervelet humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

14946-1-AP cible RPS27A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS27A Protéine recombinante Ag6750
• Nom complet: ribosomal protein S27a
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 9 kDa, 18 kDa
• Numéro d’acquisition GenBank: BC001392
• Symbole du gène: RPS27A
• Identification du gène (NCBI): 6233
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPS27A, also named as UBA80 and UBCEP1, can be cleaved into two chains Ubiquitin and 40S ribosomal protein S27a. Ubiquitin is a highly conserved 76-amino acid protein that plays a key role in protein degradation. Ubiquitin is synthesized as precursor proteins that consist either of polyubiquitin chains that are cleaved into moieties of the UBB or UBC types, or single ubiquitin moieties fused 5-prime to unrelated carboxyl extension proteins (UBA type) such as UBA52 and UBA80 (RPS27A) [PMID:10772958]

Publications

Species	Application	Title
human	WB	Mediators Inflamm; UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer.; Authors - Ruochen Zhang
human	WB	PLoS One; Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.; Authors - Yuan Tian
mouse	IF	Cell Rep; The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation; Authors - Nicolas Curdy
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren