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  • Western Blot (WB) analysis of various lysates using C2orf69 Polyclonal antibody (32100-1-AP)

    WB analysis using 32100-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32100-1-AP (C2orf69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using C2orf69 Polyclonal antibody (32100-1-AP)

    IHC staining of mouse kidney using 32100-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32100-1-AP (C2orf69 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C2orf69 Polyclonal antibody

C2orf69 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32100-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Chromosome 2 Open Reading Frame 69, COXPD53, FLJ38973, Hypothetical Protein FLJ38973, Mitochondrial protein C2orf69

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse stomach tissue, rat stomach tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32100-1-AP targets C2orf69 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2orf69 fusion protein Ag36779 Predict reactive species
Full Name chromosome 2 open reading frame 69
Calculated Molecular Weight43 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC036456
Gene Symbol C2orf69
Gene ID (NCBI) 205327
RRIDAB_3670190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8N8R5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromosome 2 open reading frame 69 (C2orf69, also known as COXPD53) is located in the mitochondrion. It can affect mitochondrial membrane potential and oxidative respiration in cultured neurons and controls the levels of the glycogen branching enzyme 1 (GBE1) (PMID: 34038740; 33945503). The variant in C2orf69 probably causes developmental regression, seizures, microcephaly, autistic features, and hypertonia (PMID: 37337918). Its deficiency can disrupt the development and homeostasis of the immune and central nervous systems (PMID: 34038740).

Protocols

Product Specific Protocols
WB protocol for C2orf69 antibody 32100-1-APDownload protocol
IHC protocol for C2orf69 antibody 32100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols