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  • Western Blot (WB) analysis of various lysates using CLN5 Recombinant antibody (85378-1-RR)

    WB analysis using 85378-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85378-1-RR (CLN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CLN5 Recombinant antibody (85378-1-RR)

    IF Staining of A431 using 85378-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLN5 antibody (85378-1-RR, Clone: 242812H6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CLN5 Recombinant antibody

CLN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85378-1-RR
Clone No.242812H6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Ceroid lipofuscinosis neuronal protein 5, BMPS, BMP synthase CLN5, Bis(monoacylglycero)phosphate synthase CLN5, secreted form, Bis(monoacylglycero)phosphate synthase CLN5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, A549 cells, HEK-293T cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85378-1-RR targets CLN5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLN5 fusion protein Ag18175 Predict reactive species
Full Name ceroid-lipofuscinosis, neuronal 5
Calculated Molecular Weight 407 aa, 46 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC153154
Gene Symbol CLN5
Gene ID (NCBI) 1203
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75503
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CLN5 (ceroid lipofuscinosis neuronal protein 5) protein is ubiquitously expressed in the majority of tissues studied and in the brain, CLN5 shows both neuronal and glial cell expression. CLN5 plays a role in maintaining an acidic environment in the lysosomes, a critical feature for a functional lysosome (PMID: 38236309). Moreover, CLN5-deficient neuronal progenitor cells showed reduced thioesterase activity, confirming the live cell function of CLN5 in setting S-depalmitoylation levels (PMID: 35427157).

Protocols

Product Specific Protocols
WB protocol for CLN5 antibody 85378-1-RRDownload protocol
IF protocol for CLN5 antibody 85378-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols