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Recombinant Mouse IL-1RA protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0685

Validation Data Gallery

  • Purity of Recombinant Mouse IL-1RA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-1RA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse IL-1RA protein Arg8-Gln159 (Accession# Q542W1) with a His tag at the C-terminus.
GeneID 16181
Accession Q542W1
PredictedSize 18.4 kDa
SDS-PAGE 16-21 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-1Ra, also known as IL-1F3, is a member of the interleukin 1 cytokine family. It is a receptor antagonist of IL-1, can competitively bind with IL-1R1 thereby blocking cell activation by the cytokine. IL-1Ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. As a potent immune/inflammatory molecule, IL-1Ra is tightly associated with osteomyelitis, rheumatoid arthritis, traumatic brain injury (TBI) and stroke. Recently, an increasing number of studies proved that IL-1Ra is also actively involved in tumor progression, such as high expression IL-1Ra increased the risk of bladder cancer and non-cardia gastric carcinoma.

References:

1. Stephane Perrier.et al. (2006) FEBS Lett. 580(27):6289-94.
2. Charles A. Dinarello. et al. (2020) Immunol Rev. 281(1):8-27.
3. Ivona Aksentijevich. et al. (2009) N Engl J Med. 360(23):2426-37.
4. C Grond-Ginsbach. et al. (2008) J Neurol. 255(5):723-31.
5. Gifone Aguiar Rocha. et al. (2005) Int J Cancer. 115(5):678-83.
6. Zhibin Hu. et al. (2006) Cancer Lett. 236(2):269-75.