Recombinant Rat MMP-8 protein (rFc Tag)
Species
Rat
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3278
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Rat MMP-8 protein Leu21-Pro466 (Accession# O88766) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 63849 |
| Accession | O88766 |
| PredictedSize | 77.2 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Matrix metalloproteinase-8 (MMP-8) is a zinc-dependent endopeptidase that plays a crucial role in various biological processes, including tissue repair, inflammation, and neurodegenerative diseases. In rats, MMP-8 has been extensively studied, and several key findings have emerged. Recent evidence suggests that reactive astrocytes play an important role in neuroinflammation and neurodegenerative diseases. A study investigated whether an MMP-8 inhibitor (M8I) could control neuroinflammation in lipoteichoic acid (LTA)-stimulated rat primary astrocytes. The results showed that M8I significantly inhibited LTA-induced expression of inflammatory molecules such as iNOS, TNF-α, IL-1β, IL-6, and TLR-2. Additionally, M8I increased the expression of phase II antioxidant enzymes such as hemeoxygenase-1, NQO1, catalase, and MnSOD by modulating the Nrf2/ARE signaling pathway. These findings suggest the therapeutic potential of an MMP-8 inhibitor in neuroinflammatory disorders associated with astrocyte reactivity.
References:
1. Yu J, Mursu E, et al. (2019) Arch Oral Biol. 97:238-244. 2. Tsubota M, Sasano Y, et al. (2002) J Dent Res. 81(10):673-8. 3. Lee EJ, Park JS, et al. (2018) J Neuroinflammation. 15(1):326. 4. Cederqvist K, et al. (2006) Pediatr Res. 60(4):395-400.