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CPSF7 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human, mouse

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 19.5-1250 pg/mL

Cat no : MP01956-1

Synonyms

Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01956-1, Human CPSF7 Recombinant Matched Antibody Pair - PBS only. 85203-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01255. 85203-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

    Sandwich ELISA standard curve of MP01956-1

    Sandwich ELISA standard curve of MP01956-1, Human CPSF7 Recombinant Matched Antibody Pair - PBS only. 85203-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01255. 85203-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • The mean CPSF7 concentration was determined to be 4,199.1 pg/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load and 1,800.3 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

    Sample test of MP01956-1

    The mean CPSF7 concentration was determined to be 4,199.1 pg/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load and 1,800.3 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01956-1 targets CPSF7 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 19.5-1250 pg/mL (Sandwich ELISA);

Capture antibody: CPSF7 Recombinant antibody, PBS Only (Capture) 85203-1-PBS (242644E1). 100 μg. Concentration 1 mg/ml.

Detection antibody: CPSF7 Recombinant antibody, PBS Only (Detector) 85203-2-PBS (242644F11). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: SY01255

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 85203-1-PBS
Tested Reactivity human, mouse
Immunogen Peptide
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name pre-mRNA cleavage factor I, 59 kDa subunit
Synonyms Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59
GenBank accession numberNM_001136040
Gene symbol CPSF7
UniprotQ8N684
Gene ID (NCBI) 79869
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 85203-2-PBS
Tested Reactivity human
Immunogen Peptide
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name pre-mRNA cleavage factor I, 59 kDa subunit
Synonyms Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59
GenBank accession numberNM_001136040
Gene symbol CPSF7
UniprotQ8N684
Gene ID (NCBI) 79869
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.