CPSF7 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85203-1-RR (same clone as 85203-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85203-1-RR (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85203-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01956-1

  Sandwich ELISA standard curve of MP01956-1, Human CPSF7 Recombinant Matched Antibody Pair - PBS only. 85203-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01255. 85203-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01956-1

  The mean CPSF7 concentration was determined to be 4,199.1 pg/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load and 1,800.3 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  IF Staining of A431 using 85203-1-RR (same clone as 85203-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (85203-1-RR, Clone: 242644E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85203-1-PBS in a different storage buffer formulation.

CPSF7 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85203-1-PBS

Clone No.242644E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59

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Product Information

85203-1-PBS targets CPSF7 as part of a matched antibody pair:

MP01956-1: 85203-1-PBS capture and 85203-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: pre-mRNA cleavage factor I, 59 kDa subunit
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_001136040
• Gene Symbol: CPSF7
• Gene ID (NCBI): 79869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N684
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.