ChromoTek 3xDYKDDDDK-peptide

Validation Data Gallery

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  DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

HPLC-purified 3xDYKDDDDK-peptide

Cat No. fp

Publications(1)

Peptide

DYKDDDDK tag

Applications

Elution

Conjugate

Unconjugated

Synonyms

DYKDDDDK peptide, DYKDDDDK, Flag

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Product Information

HPLC-purified 3xDYKDDDDK-peptide

• Description: 3xDYKDDDDK-peptide, also known as 3xFlag®-peptide
• Applications: Mild elution of DYKDDDDK-tagged fusion proteins bound to DYKDDDDK Fab-Trap or anti-DYKDDDDK antibodies.
• Sequence: MDYKDHDG DYKDHDI DYKDDDDK
• Form: Lyophilized powder
• Conjugate: Unconjugated
• Molecular weight: 2,862 g/mol
• Purity: >96% (HPLC)
• Reconstitution: Dissolve 1 mg 3xDYKDDDDK-peptide in 0.5 mL PBS pH 7.4 to a final concentration of 2 mg/mL (0.7 mM). Vortex for a few minutes to dissolve the powder. Aliquot upon reconstitution.
• Dilution: Dilute 3xDYKDDDDK-peptide stock to 150 µg/mL (0.05 mM) in PBS for elution of DYKDDDDK-tagged fusion proteins.
• Storage Condition: Upon receipt store at -20°C/-4°F. ; Aliquot upon reconstitution and store at -20°C/-4°F. ; Avoid repeated freeze / thaw cycles.
• Stability: Powder stable for 1 year upon receipt
• Shipment: Shipped at ambient temperature

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Publications

Application	Title
CoIP	Curr Biol; Evolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria; Authors - Jovelyn Unay