P glycoprotein Polyclonal antibody

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  WB analysis using 22336-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22336-1-AP (P glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 22336-1-AP

  IP result of anti-P glycoprotein (IP:22336-1-AP, 4ug; Detection:22336-1-AP 1:300) with human placenta tissue lysate 4000ug.

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  IHC staining of human liver cancer using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP (P glycoprotein antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP (P glycoprotein antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22336-1-AP (P glycoprotein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

P glycoprotein Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 22336-1-AP

Publications(111)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

ABC20, ABCB1, CD243, CLCS, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein, P glycoprotein 1, P-gp

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Tested Applications

• Positive WB detected in: unboiled HeLa cells, unboiled HepG2 cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Boiling the sample after lysis is not recommended.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 98 publications below
• IHC: See 19 publications below
• IF: See 13 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Product Information

22336-1-AP targets P glycoprotein in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P glycoprotein fusion protein Ag17811
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 1
• Calculated Molecular Weight: 1280 aa, 142 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC130424
• Gene Symbol: P glycoprotein
• Gene ID (NCBI): 5243
• RRID: AB_2833023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08183
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.

Publications

Species	Application	Title
human	WB	Drug Resist Updat; Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer; Authors - Wei Zhang
human,mouse	WB,IF	Drug Resist Updat; Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestration; Authors - Ning Ji
human	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	J Control Release; cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer; Authors - Xiaomei Liang
human	WB	ACS Appl Mater Interfaces; TPGS-Galactose-Modified Polydopamine Co-delivery Nanoparticles of Nitric Oxide Donor and Doxorubicin for Targeted Chemo-Photothermal Therapy against Drug-Resistant Hepatocellular Carcinoma.; Authors - Zijing Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SABRINA (Verified Customer) (12-07-2021)

Good antibody, but sometimes it detects also aspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: lung and breast cancer cells

P glycoprotein Antibody Western Blot validation (1:1000 dilution) in lung and breast cancer cells (Cat no:22336-1-AP)

FH

Yu Siong (Verified Customer) (03-01-2019)

It worked perfectly for my porcine brain endothelial cells!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine brain endothelial cells

P glycoprotein Antibody Western Blot, validation (1:500 dilution) in Porcine brain endothelial cells (Cat no:22336-1-AP)