P glycoprotein Polyclonal antibody
P glycoprotein Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 22336-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | unboiled HeLa cells |
Positive IP detected in | human placenta tissue |
Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 54 publications below |
IHC | See 9 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
FC | See 2 publications below |
Product Information
The immunogen of 22336-1-AP is P glycoprotein Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | P glycoprotein fusion protein Ag17811 |
Full Name | ATP-binding cassette, sub-family B (MDR/TAP), member 1 |
Calculated molecular weight | 1280 aa, 142 kDa |
Observed molecular weight | 130-150 kDa |
GenBank accession number | BC130424 |
Gene symbol | ABCB1 |
Gene ID (NCBI) | 5243 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for P glycoprotein antibody 22336-1-AP | Download protocol |
IHC protocol for P glycoprotein antibody 22336-1-AP | Download protocol |
IP protocol for P glycoprotein antibody 22336-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer. | ||
J Colloid Interface Sci DNAzyme-Based nanoflowers for reversing P-glycoprotein-mediated multidrug resistance in breast cancer | ||
Environ Pollut Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium. | ||
Eur J Med Chem Design, synthesis and bioactivity study on 5-phenylfuran derivatives as potent reversal agents against P-glycoprotein-mediated multidrug resistance in MCF-7/ADR cell. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH SABRINA (Verified Customer) (12-07-2021) | Good antibody, but sometimes it detects also aspecific bands.
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FH Yu Siong (Verified Customer) (03-01-2019) | It worked perfectly for my porcine brain endothelial cells!
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