P glycoprotein Polyclonal antibody

P glycoprotein Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22336-1-AP

Synonyms

ABC20, ABCB1, CD243, CLCS, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein, P glycoprotein 1, P-gp



Tested Applications

Positive WB detected inunboiled HeLa cells, unboiled HepG2 cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

22336-1-AP targets P glycoprotein in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P glycoprotein fusion protein Ag17811
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 1
Calculated molecular weight 1280 aa, 142 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC130424
Gene symbol ABCB1
Gene ID (NCBI) 5243
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for P glycoprotein antibody 22336-1-APDownload protocol
IHC protocol for P glycoprotein antibody 22336-1-APDownload protocol
IP protocol for P glycoprotein antibody 22336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Drug Resist Updat

Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer

Authors - Wei Zhang
humanWB,IHC

Mol Cancer

Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.

Authors - Jianyou Gu
humanWB

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

J Control Release

cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer

Authors - Xiaomei Liang
humanWB

ACS Appl Mater Interfaces

TPGS-Galactose-Modified Polydopamine Co-delivery Nanoparticles of Nitric Oxide Donor and Doxorubicin for Targeted Chemo-Photothermal Therapy against Drug-Resistant Hepatocellular Carcinoma.

Authors - Zijing Du
humanWB

ACS Appl Mater Interfaces

PMID: 31718159

Authors - Tengfei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

SABRINA (Verified Customer) (12-07-2021)

Good antibody, but sometimes it detects also aspecific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: lung and breast cancer cells
P glycoprotein Antibody Western Blot validation (1:1000 dilution) in lung and breast cancer cells (Cat no:22336-1-AP)
FH

Yu Siong (Verified Customer) (03-01-2019)

It worked perfectly for my porcine brain endothelial cells!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Porcine brain endothelial cells
P glycoprotein Antibody Western Blot, validation (1:500 dilution) in Porcine brain endothelial cells (Cat no:22336-1-AP)