|Positive WB detected in||unboiled HeLa cells, unboiled HepG2 cells|
|Positive IP detected in||human placenta tissue|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
22336-1-AP targets P glycoprotein in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||P glycoprotein fusion protein Ag17811|
|Full Name||ATP-binding cassette, sub-family B (MDR/TAP), member 1|
|Calculated molecular weight||1280 aa, 142 kDa|
|Observed molecular weight||130-150 kDa|
|GenBank accession number||BC130424|
|Gene ID (NCBI)||5243|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SABRINA (Verified Customer) (12-07-2021)
Good antibody, but sometimes it detects also aspecific bands.
Yu Siong (Verified Customer) (03-01-2019)
It worked perfectly for my porcine brain endothelial cells!