P glycoprotein Polyclonal antibody

P glycoprotein Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

59

Reviews (2)

Cat no : 22336-1-AP

Synonyms

ABC20, ABCB1, CD243, CLCS, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein, P glycoprotein 1, P-gp

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Validation Data Gallery

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  WB analysis using 22336-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22336-1-AP (P glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 22336-1-AP

  IP Result of anti-P glycoprotein (IP:22336-1-AP, 4ug; Detection:22336-1-AP 1:300) with human placenta tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP (P glycoprotein antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP (P glycoprotein antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22336-1-AP (P glycoprotein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: unboiled HeLa cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 54 publications below
• IHC: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Product Information

The immunogen of 22336-1-AP is P glycoprotein Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P glycoprotein fusion protein Ag17811
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 1
• Calculated molecular weight: 1280 aa, 142 kDa
• Observed molecular weight: 130-150 kDa
• GenBank accession number: BC130424
• Gene symbol: ABCB1
• Gene ID (NCBI): 5243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.

Publications

Species	Application	Title
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
human	WB	ACS Appl Mater Interfaces; PMID: 31718159; Authors - Tengfei Wang
human,mouse	WB,IHC	J Colloid Interface Sci; DNAzyme-Based nanoflowers for reversing P-glycoprotein-mediated multidrug resistance in breast cancer; Authors - Linna Liang
human	WB	Environ Pollut; Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium.; Authors - Jianqiang Zhu
human	WB	Eur J Med Chem; Design, synthesis and bioactivity study on 5-phenylfuran derivatives as potent reversal agents against P-glycoprotein-mediated multidrug resistance in MCF-7/ADR cell.; Authors - Ya-Sheng Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SABRINA (Verified Customer) (12-07-2021)

Good antibody, but sometimes it detects also aspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: lung and breast cancer cells

P glycoprotein Antibody Western Blot validation (1:1000 dilution) in lung and breast cancer cells (Cat no:22336-1-AP)

FH

Yu Siong (Verified Customer) (03-01-2019)

It worked perfectly for my porcine brain endothelial cells!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine brain endothelial cells

P glycoprotein Antibody Western Blot, validation (1:500 dilution) in Porcine brain endothelial cells (Cat no:22336-1-AP)

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