ACAA1 Antibody

ACAA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12319-2-AP

Synonyms

ACAA, ACAA1, Acetyl CoA acyltransferase, Beta ketothiolase, PTHIO, THIO



Tested Applications

Positive WB detected inL02 cells, A549 cells, human lung tissue
Positive IHC detected inhuman thyroid cancer tissue, human liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

12319-2-AP targets ACAA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAA1 fusion protein Ag2972
Full Name acetyl-Coenzyme A acyltransferase 1
Calculated molecular weight 424 aa, 44 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC011977
Gene symbol ACAA1
Gene ID (NCBI) 30
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACAA1 antibody 12319-2-APDownload protocol
IHC protocol for ACAA1 antibody 12319-2-APDownload protocol
IF protocol for ACAA1 antibody 12319-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

J Agric Food Chem

Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.

Authors - Chaochao Luo
mouseIHC

Biochimie

In vivo labeling of peroxisomes by photoconvertible mEos2 in myelinating glia of mice.

Authors - Richert Sarah S
mouseWB,IHC

FEBS Lett

A role for myelin-associated peroxisomes in maintaining paranodal loops and axonal integrity.

Authors - Kassmann Celia M CM
humanWB

Int J Mol Sci

PMID: 32156004

Authors - Lei Xu
humanIF

Sci Rep

Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes.

Authors - Kareem Soliman
ratWB

EBioMedicine

PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

Authors - Zhongshi Lyu