ACAA1 Antibody

ACAA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12319-2-AP

Synonyms

ACAA, ACAA1, Acetyl CoA acyltransferase, Beta ketothiolase, PTHIO, THIO

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Validation Data Gallery

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  IF staining of U-251 cells using 12319-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 12319-2-AP (ACAA1 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  WB analysis of L02 cells using 12319-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12319-2-AP (ACAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer tissue using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human thyroid cancer tissue using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells, A549 cells, human lung tissue
• Positive IHC detected in: human thyroid cancer tissue, human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:250-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

12319-2-AP targets ACAA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAA1 fusion protein Ag2972
• Full Name: acetyl-Coenzyme A acyltransferase 1
• Calculated molecular weight: 424 aa, 44 kDa
• Observed molecular weight: 41 kDa
• GenBank accession number: BC011977
• Gene symbol: ACAA1
• Gene ID (NCBI): 30
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
	IF	J Agric Food Chem; Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.; Authors - Chaochao Luo
mouse	IHC	Biochimie; In vivo labeling of peroxisomes by photoconvertible mEos2 in myelinating glia of mice.; Authors - Richert Sarah S
mouse	WB,IHC	FEBS Lett; A role for myelin-associated peroxisomes in maintaining paranodal loops and axonal integrity.; Authors - Kassmann Celia M CM
human	WB	Int J Mol Sci; PMID: 32156004; Authors - Lei Xu
human	IF	Sci Rep; Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes.; Authors - Kareem Soliman
rat	WB	EBioMedicine; PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.; Authors - Zhongshi Lyu