ACAA1 Polyclonal antibody

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  WB analysis of L02 using 12319-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12319-2-AP (ACAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12319-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12319-2-AP (ACAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12319-2-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12319-2-AP (ACAA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human thyroid cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12319-2-AP (ACAA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12319-2-AP (ACAA1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of U-251 using 12319-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 12319-2-AP (ACAA1 antibody), at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ACAA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12319-2-AP

Publications(18)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Acetyl CoA acyltransferase, ACAA, 3-ketoacyl-CoA thiolase, peroxisomal

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Tested Applications

• Positive WB detected in: L02 cells, A549 cells, human lung tissue
• Positive IHC detected in: human thyroid cancer tissue, human lung tissue, human prostate cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Product Information

12319-2-AP targets ACAA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAA1 fusion protein Ag2972
• Full Name: acetyl-Coenzyme A acyltransferase 1
• Calculated Molecular Weight: 424 aa, 44 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC011977
• Gene Symbol: ACAA1
• Gene ID (NCBI): 30
• RRID: AB_2289045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09110
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).

Publications

Species	Application	Title
human	IF	Drug Resist Updat; MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance; Authors - GuoSheng Wang
human	IF	Hum Mol Genet; Functional analysis of PEX13 mutation in a Zellweger syndrome spectrum patient reveals novel homooligomerization of PEX13 and its role in human peroxisome biogenesis.; Authors - Krause Cindy C
human	WB	Cell Mol Life Sci; Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis.; Authors - Pablo Ranea-Robles
rat	WB	EBioMedicine; PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.; Authors - Zhongshi Lyu
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
	IF	J Agric Food Chem; Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.; Authors - Chaochao Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PAULA (Verified Customer) (07-12-2023)

I tested this ACAA1 antibody on THP1 cells (20 ug total cell lysate) and two nice bands were observed at the most concentrated dilution (1:250), although they were located slightly below the predicted molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500, 1:250
• Cell Tissue Type: THP1 cell line

ACAA1 Antibody Western Blot validation (1:500, 1:250 dilution) in THP1 cell line (Cat no:12319-2-AP)

FH

Tom (Verified Customer) (04-01-2022)

1:1000 for 18 hours at 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cos7

ACAA1 Antibody Western Blot validation (1:1000 dilution) in Cos7 (Cat no:12319-2-AP)