ACAA1 Polyclonal antibody
ACAA1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12319-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, A549 cells, human lung tissue |
Positive IHC detected in | human thyroid cancer tissue, human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U-251 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:250-1:1000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
Product Information
12319-2-AP targets ACAA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACAA1 fusion protein Ag2972 |
Full Name | acetyl-Coenzyme A acyltransferase 1 |
Calculated molecular weight | 424 aa, 44 kDa |
Observed molecular weight | 41 kDa |
GenBank accession number | BC011977 |
Gene symbol | ACAA1 |
Gene ID (NCBI) | 30 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for ACAA1 antibody 12319-2-AP | Download protocol |
IHC protocol for ACAA1 antibody 12319-2-AP | Download protocol |
IF protocol for ACAA1 antibody 12319-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Agric Food Chem Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation. | ||
Biochimie In vivo labeling of peroxisomes by photoconvertible mEos2 in myelinating glia of mice. | ||
FEBS Lett A role for myelin-associated peroxisomes in maintaining paranodal loops and axonal integrity. | ||
Sci Rep Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes. | ||
EBioMedicine PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules. |