Validation Data Gallery
|Positive WB detected in
|L02 cells, A549 cells, human lung tissue
|Positive IHC detected in
|human thyroid cancer tissue, human lung tissue, human prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:20-1:200
|IF : 1:250-1:1000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12319-2-AP targets ACAA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ACAA1 fusion protein Ag2972
|acetyl-Coenzyme A acyltransferase 1
|Calculated molecular weight
|424 aa, 44 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).
Hum Mol Genet
Functional analysis of PEX13 mutation in a Zellweger syndrome spectrum patient reveals novel homooligomerization of PEX13 and its role in human peroxisome biogenesis.
Cell Mol Life Sci
Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis.
PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.
Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism
J Agric Food Chem
Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.
Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
PAULA (Verified Customer) (07-12-2023)
I tested this ACAA1 antibody on THP1 cells (20 ug total cell lysate) and two nice bands were observed at the most concentrated dilution (1:250), although they were located slightly below the predicted molecular weight.
Tom (Verified Customer) (04-01-2022)
1:1000 for 18 hours at 4 degrees