ACAA2 Polyclonal antibody

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  WB analysis using 11111-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11111-1-AP (ACAA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11111-1-AP

  WB result of ACAA2 antibody (11111-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAA2 transfected HEK-293 cells.

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  IHC staining of human lung cancer using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ACAA2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11111-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

Beta ketothiolase, Acyl-CoA hydrolase, mitochondrial, Acetyl-CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl CoA acyltransferase

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human colon tissue, human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 2 publications below
• IP: See 2 publications below

Product Information

11111-1-AP targets ACAA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1609

  Product name: Recombinant human ACAA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 98-397 aa of BC001918

  Sequence: SIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA

• Full Name: acetyl-Coenzyme A acyltransferase 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC001918
• Gene Symbol: ACAA2
• Gene ID (NCBI): 10449
• RRID: AB_2219389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42765
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncotarget; Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein.; Authors - Yanxiao Zhang
rat	WB	EBioMedicine; PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.; Authors - Zhongshi Lyu
rat	WB,IHC	PLoS One; Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.; Authors - Baandrup Jonas D JD
sheep	WB	Front Genet; Identification of miRNA in Sheep Intramuscular Fat and the Role of miR-193a-5p in Proliferation and Differentiation of 3T3-L1.; Authors - Fuhui Han
rat	WB	Gerontology; Dietary Restriction Increases Protein Acetylation in the Livers of Aged Rats.; Authors - Nakamura Akihiro A
human	WB	J Biol Chem; CLPX regulates mitochondrial fatty acid β-oxidation in liver cells; Authors - Ko Suzuki