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ACC1 Antibody 19 Publications

Rabbit Polyclonal| Catalog number: 21923-1-AP

Featured Product KD/KO validated

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ACC1 Antibody WB HeLa cells 21923-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 21923-1-AP(ACACA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ACC1 Antibody IP HeLa cells 21923-1-AP

IP Result of anti-ACC1 (IP:21923-1-AP, 5ug; Detection:21923-1-AP 1:500) with HeLa cells lysate 2800ug.

ACC1 Antibody IHC human skeletal muscle tissue 21923-1-AP

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 21923-1-AP( ACC1 antibody at dilution of 1:200 (under 10x lens).

ACC1 Antibody IHC human skeletal muscle tissue 21923-1-AP

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 21923-1-AP( ACC1 antibody at dilution of 1:200 (under 40x lens).

ACC1 Antibody IF HeLa cells 21923-1-AP

Immunofluorescent analysis of HeLa cells using 21923-1-AP(ACC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, rat, pig
Positive WB detected in:
HeLa cells, HEK-293 cells, human brain tissue, PC-3 cells, rat brain tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human skeletal muscle tissue, human kidney tissue, human renal cell carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACC1 fusion protein Ag16452
Full name:
acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight:
2383aa,275 kDa
Observed molecular weight:
260-270 kDa
GenBank accession number:
BC137287
Gene ID (NCBI):
31
Gene symbol
ACACA
Synonyms
ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

Background

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ACC1 Rabbit Polyclonal antibody

Kishor (Verified Customer), 02-22-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
ACC1 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:21923-1-AP)

ACC1 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:21923-1-AP)

Lot Number
I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be

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