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ACC1 Antibody 18 Publications

Rabbit Polyclonal| Catalog number: 21923-1-AP

Featured Product KD/KO validated

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Con: 79 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ACC1 Antibody WB HeLa cells 21923-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 21923-1-AP(ACACA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ACC1 Antibody IP HeLa cells 21923-1-AP

IP Result of anti-ACC1 (IP:21923-1-AP, 5ug; Detection:21923-1-AP 1:500) with HeLa cells lysate 2800ug.

ACC1 Antibody IHC human skeletal muscle tissue 21923-1-AP

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 21923-1-AP( ACC1 antibody at dilution of 1:200 (under 10x lens).

ACC1 Antibody IHC human skeletal muscle tissue 21923-1-AP

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 21923-1-AP( ACC1 antibody at dilution of 1:200 (under 40x lens).

ACC1 Antibody IF HeLa cells 21923-1-AP

Immunofluorescent analysis of HeLa cells using 21923-1-AP(ACC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, rat, pig
Positive WB detected in:
HeLa cells, HEK-293 cells, human brain tissue, PC-3 cells, rat brain tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human skeletal muscle tissue, human kidney tissue, human renal cell carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACC1 fusion protein Ag16452
Full name:
acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight:
2383aa,275 kDa
Observed molecular weight:
260-270 kDa
GenBank accession number:
BC137287
Gene ID (NCBI):
31
Gene symbol
ACACA
Synonyms
ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

Background

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.


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