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- KD/KO Validated
ACC1 Polyclonal antibody
ACC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21923-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, PC-3 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human skeletal muscle tissue, human kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 39 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 21923-1-AP is ACC1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACC1 fusion protein Ag16452 |
| Full Name | acetyl-Coenzyme A carboxylase alpha |
| Calculated molecular weight | 2383 aa, 275 kDa |
| Observed molecular weight | 260-270 kDa |
| GenBank accession number | BC137287 |
| Gene symbol | ACACA |
| Gene ID (NCBI) | 31 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACC1 antibody 21923-1-AP | Download protocol |
| IHC protocol for ACC1 antibody 21923-1-AP | Download protocol |
| IF protocol for ACC1 antibody 21923-1-AP | Download protocol |
| IP protocol for ACC1 antibody 21923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioconjug Chem Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.
| ||
Biochem Biophys Res Commun microRNA-16-5p promotes 3T3-L1 adipocyte differentiation through regulating EPT1. | ||
Onco Targets Ther MEX3C regulates lipid metabolism to promote bladder tumorigenesis through JNK pathway. | ||
Animals (Basel) Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs. | ||
Biochim Biophys Acta Gestational dietary betaine supplementation suppresses hepatic expression of lipogenic genes in neonatal piglets through epigenetic and glucocorticoid receptor-dependent mechanisms. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kishor (Verified Customer) (02-22-2019) | I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be
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