ACC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACC1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

75

Reviews (1)

Cat no : 21923-1-AP

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

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Validation Data Gallery

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  WB analysis using 21923-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21923-1-AP (ACC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21923-1-AP

  WB result of ACC1 antibody (21923-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACC1 transfected HeLa cells.

• 

  IP experiment of HeLa using 21923-1-AP

  IP Result of anti-ACC1 (IP:21923-1-AP, 5ug; Detection:21923-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of mouse skeletal muscle using 21923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21923-1-AP (ACC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 21923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21923-1-AP (ACC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21923-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21923-1-AP (ACACA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21923-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21923-1-AP (ACACA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 21923-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21923-1-AP (ACACA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21923-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21923-1-AP (ACACA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21923-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ACC1 antibody (21923-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 21923-1-AP

  Immunofluorescent analysis of HeLa cells using 21923-1-AP (ACC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21923-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ACC1 (21923-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse skeletal muscle tissue, human kidney tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 67 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Product Information

The immunogen of 21923-1-AP is ACC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat, bovine, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACC1 fusion protein Ag16452
• Full Name: acetyl-Coenzyme A carboxylase alpha
• Calculated molecular weight: 2383 aa, 275 kDa
• Observed molecular weight: 250 kDa
• GenBank accession number: BC137287
• Gene symbol: ACACA
• Gene ID (NCBI): 31
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Publications

Species	Application	Title
human	WB	Bioconjug Chem; Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.; Authors - Yipeng Du ; KD Validated
mouse	WB	Biochem Biophys Res Commun; microRNA-16-5p promotes 3T3-L1 adipocyte differentiation through regulating EPT1.; Authors - Jingjing Xu
human	WB,IHC	Onco Targets Ther; MEX3C regulates lipid metabolism to promote bladder tumorigenesis through JNK pathway.; Authors - Haichao Chao
	WB	Animals (Basel); Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs.; Authors - Chaogang Yao
human	WB	Oncotarget; PMID: 28052008; Authors - Chaojun Xue
pig	WB	Biochim Biophys Acta; Gestational dietary betaine supplementation suppresses hepatic expression of lipogenic genes in neonatal piglets through epigenetic and glucocorticoid receptor-dependent mechanisms.; Authors - Demin Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (02-22-2019)

I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000

ACC1 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:21923-1-AP)

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