• Featured Product
  • KD/KO Validated

ACC1 Polyclonal antibody

ACC1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21923-1-AP

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue, human kidney tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21923-1-AP is ACC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, bovine, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACC1 fusion protein Ag16452
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight 2383 aa, 275 kDa
Observed molecular weight 250 kDa
GenBank accession numberBC137287
Gene symbol ACACA
Gene ID (NCBI) 31
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Protocols

Product Specific Protocols
WB protocol for ACC1 antibody 21923-1-APDownload protocol
IHC protocol for ACC1 antibody 21923-1-APDownload protocol
IF protocol for ACC1 antibody 21923-1-APDownload protocol
IP protocol for ACC1 antibody 21923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bioconjug Chem

Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.

Authors - Yipeng Du
  • KD Validated
mouseWB

Biochem Biophys Res Commun

microRNA-16-5p promotes 3T3-L1 adipocyte differentiation through regulating EPT1.

Authors - Jingjing Xu
humanWB,IHC

Onco Targets Ther

MEX3C regulates lipid metabolism to promote bladder tumorigenesis through JNK pathway.

Authors - Haichao Chao
WB

Animals (Basel)

Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs.

Authors - Chaogang Yao
humanWB

Oncotarget

PMID: 28052008

Authors - Chaojun Xue
pigWB

Biochim Biophys Acta

Gestational dietary betaine supplementation suppresses hepatic expression of lipogenic genes in neonatal piglets through epigenetic and glucocorticoid receptor-dependent mechanisms.

Authors - Demin Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kishor (Verified Customer) (02-22-2019)

I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
ACC1 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:21923-1-AP)