ACADL Polyclonal antibody

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  WB analysis using 17442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17442-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17442-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17442-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17442-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17442-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

ACADL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17442-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ACAD4, ACADL, LCAD

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HepG2 cells, rat kidney tissue, HeLa cells, HEK-293 cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

17442-1-AP targets ACADL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11530

  Product name: Recombinant human ACADL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 33-430 aa of BC039063

  Sequence: EERLETPSAKKLTDIGIRRIFSPEHDIFRKSVRKFFQEEVIPHHSEWEKAGEVSREVWEKAGKQGLLGVNIAEHLGGIGGDLYSAAIVWEEQAYSNCSGPGFSIHSGIVMSYITNHGSEEQIKHFIPQMTAGKCIGAIAMTEPGAGSDLQGIKTNAKKDGSDWILNGSKVFISNGSLSDVVIVVAVTNHEAPSPAHGISLFLVENGMKGFIKGRKLHKMGLKAQDTAELFFEDIRLPASALLGEENKGFYYIMKELPQERLLIADVAISASEFMFEETRNYVKQRKAFGKTVAHLQTVQHKLAELKTHICVTRAFVDNCLQLHEAKRLDSATACMAKYWASELQNSVAYDCVQLHGGWGYMWEYPIAKAYVDARVQPIYGGTNEIMKELIAREIVFDK

• Full Name: acyl-Coenzyme A dehydrogenase, long chain
• Calculated Molecular Weight: 430 aa, 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC039063
• Gene Symbol: ACADL
• Gene ID (NCBI): 33
• RRID: AB_2219662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients.; Authors - Ya-Kun Zhang
mouse	IHC	J Transl Med; Identification and experimental validation of KMO as a critical immune-associated mitochondrial gene in unstable atherosclerotic plaque; Authors - Fu-Jun Liao
rat	WB	Front Pharmacol; Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease; Authors - Xinhui Liu
mouse	WB	Front Pharmacol; The serum acylcarnitines profile in epileptic children treated with valproic acid and the protective roles of peroxisome proliferator-activated receptor a activation in valproic acid-induced liver injury; Authors - Yiyi Ma
human,mouse	WB	Diabetologia; Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation; Authors - Shao-Wen Weng
human	WB	Cancer Metab; A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells; Authors - Shohei Matsufuji