ACADM Monoclonal antibody, PBS Only

ACADM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11G6

Cat no : 67742-1-PBS

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

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Validation Data Gallery

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  WB analysis using 67742-1-Ig (same clone as 67742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67742-1-Ig (same clone as 67742-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67742-1-Ig (same clone as 67742-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ACADM antibody (67742-1-Ig, Clone: 1A11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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Product Information

67742-1-PBS targets ACADM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, rat, pig samples.

• Tested Reactivity: Human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACADM fusion protein Ag30620
• Full Name: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Calculated Molecular Weight: 421 aa, 47 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC005377
• Gene Symbol: ACADM
• Gene ID (NCBI): 34
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.