ACADM Monoclonal antibody

ACADM Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11G6

Cat no : 67742-1-Ig

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH



Tested Applications

Positive WB detected inPC-3 cells, HSC-T6 cells, rat liver tissue
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67742-1-Ig targets ACADM in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACADM fusion protein Ag30620
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Calculated molecular weight 421 aa, 47 kDa
Observed molecular weight42 kDa
GenBank accession numberBC005377
Gene symbol ACADM
Gene ID (NCBI) 34
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Protocols

Product Specific Protocols
WB protocol for ACADM antibody 67742-1-IgDownload protocol
IHC protocol for ACADM antibody 67742-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols