ACADM Monoclonal antibody

ACADM Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, rat, pig







Cat no : 67742-1-Ig


ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

Tested Applications

Positive WB detected inPC-3 cells, HSC-T6 cells, rat liver tissue, HeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67742-1-Ig targets ACADM in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACADM fusion protein Ag30620
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Calculated Molecular Weight 421 aa, 47 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC005377
Gene Symbol ACADM
Gene ID (NCBI) 34
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.


Product Specific Protocols
WB protocol for ACADM antibody 67742-1-IgDownload protocol
IHC protocol for ACADM antibody 67742-1-IgDownload protocol
IF protocol for ACADM antibody 67742-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Mol Sci

High-Dose Fenofibrate Stimulates Multiple Cellular Stress Pathways in the Kidney of Old Rats

Authors - Agata Wrońska


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Agata (Verified Customer) (04-16-2024)

The antibody used in WB gave a single clear band, of appropriate weight, with a strong signal, in rat kidney tissue homogenates - the antibody was excellent on every try.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: rat kidney
ACADM Antibody Western Blot validation (1:5000 dilution) in rat kidney (Cat no:67742-1-Ig)