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Aggrecan Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 13880-1-AP

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Con: 39 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Aggrecan Antibody WB ROS1728 cells 13880-1-AP

ROS1728 cells were subjected to SDS PAGE followed by western blot with 13880-1-AP (Aggrecan antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Aggrecan Antibody IHC human lung tissue 13880-1-AP

Immunohistochemistry of paraffin-embedded human lung tissue slide using 13880-1-AP( Aggrecan antibody at dilution of 1:400 (under 10x lens)

Aggrecan Antibody IHC human lung tissue 13880-1-AP

Immunohistochemistry of paraffin-embedded human lung tissue slide using 13880-1-AP( Aggrecan antibody at dilution of 1:400 (under 40x lens)

Aggrecan Antibody IF A549 cells 13880-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed A549 cells using 13880-1-AP(Aggrecan antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, rabbit, zebrafish
Positive WB detected in:
ROS1728 cells
Positive IHC detected in:
human lung tissue, human cartilage tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A549 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Aggrecan fusion protein Ag4985
Full name:
aggrecan
Calculated molecular weight:
250 kDa
Observed molecular weight:
70 kDa
GenBank accession number:
BC036445
Gene ID (NCBI):
176
Gene symbol
ACAN
Synonyms
ACAN, AGC1, AGCAN, aggrecan, CSPG1, CSPGCP, MSK16, SEDK

Background

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. This polyclonal antibody raised against 1-298aa of human aggrecan reveals a band of 70 kDa, which is consistent with the molecular weight of degradation product generated by proteolysis at the aggrecanase site (PMID: 7852317; 9337874).


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