Aggrecan Polyclonal antibody

Aggrecan Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13880-1-AP

Synonyms

ACAN, AGC1, AGCAN, aggrecan, CSPG1, CSPGCP, MSK16, SEDK



Tested Applications

Positive IHC detected inhuman lung tissue, human lung cancer tissue, human cartilage tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

13880-1-AP targets Aggrecan in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, pig, rabbit, rabbits, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aggrecan fusion protein Ag4985
Full Name aggrecan
Calculated molecular weight 250 kDa
GenBank accession numberBC036445
Gene symbol ACAN
Gene ID (NCBI) 176
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Protocols

Product Specific Protocols
IHC protocol for Aggrecan antibody 13880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
humanWB

J Extracell Vesicles

miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration.

Authors - Hongxing Hu
mouseWB

Nat Commun

Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.

Authors - Mehdi Eshraghi
ratIF

Autophagy

HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy.

Authors - Ruijun He
humanIHC

Arthritis Rheumatol

Involvement of Transient Receptor Potential Vanilloid Channel 2 in the Induction of Lubricin and Suppression of Ectopic Endochondral Ossification in Mouse Articular Cartilage.

Authors - Hideki Nakamoto
mouse,ratWB,IHC,IF

Theranostics

A thermosensitive, reactive oxygen species-responsive, MR409-encapsulated hydrogel ameliorates disc degeneration in rats by inhibiting the secretory autophagy pathway.

Authors - Qiangqiang Zheng