|Positive IHC detected in||human lung tissue, human cartilage tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
13880-1-AP targets Aggrecan in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rabbit, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Aggrecan fusion protein Ag4985|
|Calculated molecular weight||250 kDa|
|GenBank accession number||BC036445|
|Gene ID (NCBI)||176|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. This polyclonal antibody raised against 1-298aa of human aggrecan reveals a band of 70 kDa, which is consistent with the molecular weight of degradation product generated by proteolysis at the aggrecanase site (PMID: 7852317; 9337874).
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