Aggrecan Polyclonal antibody
Aggrecan Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
149
Cat no : 13880-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human lung tissue, human lung cancer tissue, human cartilage tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 97 publications below |
IHC | See 48 publications below |
IF | See 35 publications below |
Product Information
13880-1-AP targets Aggrecan in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, canine, pig, rabbit, rabbits, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Aggrecan fusion protein Ag4985 |
Full Name | aggrecan |
Calculated molecular weight | 250 kDa |
GenBank accession number | BC036445 |
Gene symbol | ACAN |
Gene ID (NCBI) | 176 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Protocols
Product Specific Protocols | |
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IHC protocol for Aggrecan antibody 13880-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Extracell Vesicles miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration. | ||
Nat Commun Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease. | ||
Autophagy HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy. | ||
Arthritis Rheumatol Involvement of Transient Receptor Potential Vanilloid Channel 2 in the Induction of Lubricin and Suppression of Ectopic Endochondral Ossification in Mouse Articular Cartilage. | ||
Theranostics A thermosensitive, reactive oxygen species-responsive, MR409-encapsulated hydrogel ameliorates disc degeneration in rats by inhibiting the secretory autophagy pathway. |