Aggrecan Polyclonal antibody

Aggrecan Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

106

Cat no : 13880-1-AP

Synonyms

ACAN, AGC1, AGCAN, aggrecan, CSPG1, CSPGCP, MSK16, SEDK

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Validation Data Gallery

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  IHC staining of human lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human lung cancer using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cartilage using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cartilage tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human cartilage using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cartilage tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human lung tissue, human lung cancer tissue, human cartilage tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 69 publications below
• IHC: See 31 publications below
• IF: See 20 publications below

Product Information

13880-1-AP targets Aggrecan in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, rabbits, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aggrecan fusion protein Ag4985
• Full Name: aggrecan
• Calculated molecular weight: 250 kDa
• GenBank accession number: BC036445
• Gene symbol: ACAN
• Gene ID (NCBI): 176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; MiR-499a-5p suppresses apoptosis of human nucleus pulposus cells and degradation of their extracellular matrix by targeting SOX4.; Authors - Jing-Chuan Sun
human	WB	Bone; miR-93-5p attenuates IL-1β-induced chondrocyte apoptosis and cartilage degradation in osteoarthritis partially by targeting TCF4.; Authors - Huaming Xue
mouse	WB,IF	Theranostics; Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane.; Authors - Xiaolong Xu
mouse		Stem Cells Int; Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation.; Authors - Hai-Lin Pang
human	IHC	Biochem Biophys Res Commun; Leukemia inhibitory factor promotes extracellular matrix synthesis in degenerative nucleus pulposus cells via MAPK-ERK1/2 signaling pathway.; Authors - Hao Zhou
		Acta Biochim Biophys Sin (Shanghai); GPx1 knockdown suppresses chondrogenic differentiation of ATDC5 cells through induction of reductive stress.; Authors - Jidong Yan

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