Aggrecan Polyclonal antibody
Aggrecan Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13880-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human lung tissue, human cartilage tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 43 publications below |
| IHC | See 19 publications below |
| IF | See 14 publications below |
Product Information
13880-1-AP targets Aggrecan in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, rabbit, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Aggrecan fusion protein Ag4985 |
| Full Name | aggrecan |
| Calculated molecular weight | 250 kDa |
| GenBank accession number | BC036445 |
| Gene symbol | ACAN |
| Gene ID (NCBI) | 176 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. This polyclonal antibody raised against 1-298aa of human aggrecan reveals a band of 70 kDa, which is consistent with the molecular weight of degradation product generated by proteolysis at the aggrecanase site (PMID: 7852317; 9337874).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Aggrecan antibody 13880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother MiR-499a-5p suppresses apoptosis of human nucleus pulposus cells and degradation of their extracellular matrix by targeting SOX4. | ||
Bone miR-93-5p attenuates IL-1β-induced chondrocyte apoptosis and cartilage degradation in osteoarthritis partially by targeting TCF4. | ||
Theranostics Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane. | ||
Stem Cells Int Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation. | ||
Biochem Biophys Res Commun Leukemia inhibitory factor promotes extracellular matrix synthesis in degenerative nucleus pulposus cells via MAPK-ERK1/2 signaling pathway. | ||
Acta Biochim Biophys Sin (Shanghai) GPx1 knockdown suppresses chondrogenic differentiation of ATDC5 cells through induction of reductive stress. |
