Aggrecan Polyclonal antibody
Aggrecan Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
106
Cat no : 13880-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human lung tissue, human lung cancer tissue, human cartilage tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 69 publications below |
IHC | See 31 publications below |
IF | See 20 publications below |
Product Information
13880-1-AP targets Aggrecan in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, rabbit, rabbits, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Aggrecan fusion protein Ag4985 |
Full Name | aggrecan |
Calculated molecular weight | 250 kDa |
GenBank accession number | BC036445 |
Gene symbol | ACAN |
Gene ID (NCBI) | 176 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Protocols
Product Specific Protocols | |
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IHC protocol for Aggrecan antibody 13880-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomed Pharmacother MiR-499a-5p suppresses apoptosis of human nucleus pulposus cells and degradation of their extracellular matrix by targeting SOX4. | ||
Bone miR-93-5p attenuates IL-1β-induced chondrocyte apoptosis and cartilage degradation in osteoarthritis partially by targeting TCF4. | ||
Theranostics Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane. | ||
Stem Cells Int Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation. | ||
Biochem Biophys Res Commun Leukemia inhibitory factor promotes extracellular matrix synthesis in degenerative nucleus pulposus cells via MAPK-ERK1/2 signaling pathway. | ||
Acta Biochim Biophys Sin (Shanghai) GPx1 knockdown suppresses chondrogenic differentiation of ATDC5 cells through induction of reductive stress. |