Aggrecan Polyclonal antibody

Aggrecan Polyclonal Antibody for IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

274

Cat no : 13880-1-AP

Synonyms

ACAN, Cartilage-specific proteoglycan core protein, Aggrecan core protein 2, Aggrecan 1, AGCAN

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Validation Data Gallery

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  IHC staining of rat brain using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of rat brain using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cartilage using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cartilage tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human cartilage using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cartilage tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of mouse lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of ATDC-5 using 13880-1-AP

  1X10^6 ATDC-5 cells were intracellularly stained with 0.8 ug Anti-Human Aggrecan (13880-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IHC detected in: human lung tissue, human cartilage tissue, human lung cancer tissue, mouse lung tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: ATDC-5 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 90 publications below
• IF: See 87 publications below

Product Information

13880-1-AP targets Aggrecan in IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aggrecan fusion protein Ag4985
• Full Name: aggrecan
• Calculated Molecular Weight: 250 kDa
• GenBank Accession Number: BC036445
• Gene Symbol: ACAN
• Gene ID (NCBI): 176
• RRID: AB_2722780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Publications

Species	Application	Title
human,mouse	IHC,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human,mouse	IF	Cell Stem Cell; A human organoid drug screen identifies α2-adrenergic receptor signaling as a therapeutic target for cartilage regeneration; Authors - Xiaocui Wei
human		Sci Transl Med; Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology; Authors - Su'an Tang
human		J Extracell Vesicles; miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration.; Authors - Hongxing Hu
rat	IF	Biomaterials; Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration; Authors - Lei Liu
mouse	IHC,IF	Bone Res; Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome; Authors - Yanqiu Wang

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