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  • KD/KO Validated

ACLY Polyclonal antibody

ACLY Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15421-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

ATP citrate lyase, ACL, ATP-citrate, ATP-citrate synthase, EC:2.3.3.8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, HeLa cells, L02 cells, Jurkat cells, mouse brain tissue, rat brain tissue, K-562 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15421-1-AP targets ACLY in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7709

Product name:

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: of

Sequence:

Predict reactive species
Full Name ATP citrate lyase
Calculated Molecular Weight 121 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC006195
Gene Symbol ACLY
Gene ID (NCBI) 47
RRIDAB_2223741
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP53396
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACLY antibody 15421-1-APDownload protocol
IHC protocol for ACLY antibody 15421-1-APDownload protocol
IF protocol for ACLY antibody 15421-1-APDownload protocol
IP protocol for ACLY antibody 15421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.

Authors - Steven Zhao
mouseWB,IF

Nature

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.

Authors - Philipp Mews
mouse,humanWB

Cell

Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.

Authors - Xiaojing Liu
  • KO Validated
mouseWB

Cell Metab

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Authors - Peng Zhou
mouseWB

Mol Cell

Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation

Authors - Sophie Trefely
  • KO Validated
humanWB

Mol Cell

Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.

Authors - Sharanya Sivanand
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Uthra (Verified Customer) (01-28-2021)

Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Liver
ACLY Antibody Immunohistochemistry validation (1:200 dilution) in Liver (Cat no:15421-1-AP)
FH

K (Verified Customer) (12-20-2020)

This antibody is working good for WB (1:1000) and IHC (1:200)

  • Applications: Western Blot, Immunohistochemistry
ACLY Antibody Western Blot,Immunohistochemistry validation ( dilution) in (Cat no:15421-1-AP)
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