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ACLY Polyclonal antibody
ACLY Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15421-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, A549 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, K-562 cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 16 publications below |
WB | See 71 publications below |
IHC | See 6 publications below |
IF | See 10 publications below |
IP | See 4 publications below |
CoIP | See 4 publications below |
Product Information
15421-1-AP targets ACLY in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACLY fusion protein Ag7709 |
Full Name | ATP citrate lyase |
Calculated Molecular Weight | 121 kDa |
Observed Molecular Weight | 120 kDa |
GenBank Accession Number | BC006195 |
Gene Symbol | ACLY |
Gene ID (NCBI) | 47 |
RRID | AB_2223741 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for ACLY antibody 15421-1-AP | Download protocol |
IHC protocol for ACLY antibody 15421-1-AP | Download protocol |
IF protocol for ACLY antibody 15421-1-AP | Download protocol |
IP protocol for ACLY antibody 15421-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.
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Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate | ||
Mol Cell Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation
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Mol Cell Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Uthra (Verified Customer) (01-28-2021) | Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.
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FH K (Verified Customer) (12-20-2020) | This antibody is working good for WB (1:1000) and IHC (1:200)
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