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ACLY Polyclonal antibody
ACLY Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15421-1-AP
Validation Data Gallery
|Positive WB detected in
|L02 cells, A549 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, K-562 cells, MCF-7 cells
|Positive IP detected in
|Positive IHC detected in
|human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:10-1:100
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15421-1-AP targets ACLY in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, chicken
|Host / Isotype
|Rabbit / IgG
|ACLY fusion protein Ag7709
|ATP citrate lyase
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.
|Product Specific Protocols
|WB protocol for ACLY antibody 15421-1-AP
|IHC protocol for ACLY antibody 15421-1-AP
|IF protocol for ACLY antibody 15421-1-AP
|IP protocol for ACLY antibody 15421-1-AP
|FC protocol for ACLY antibody 15421-1-AP
|Click here to view our Standard Protocols
Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.
Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.
Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.
High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate
Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation
Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Uthra (Verified Customer) (01-28-2021)
Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.
K (Verified Customer) (12-20-2020)
This antibody is working good for WB (1:1000) and IHC (1:200)