Aconitase 1 Polyclonal antibody

Aconitase 1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12406-1-AP

Synonyms

ACO1, Aconitase, Aconitase 1, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1



Tested Applications

Positive WB detected inmouse kidney tissue, HepG2 cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12406-1-AP targets Aconitase 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aconitase 1 fusion protein Ag3083
Full Name aconitase 1, soluble
Calculated molecular weight 889 aa, 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC018103
Gene symbol ACO1
Gene ID (NCBI) 48
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Aconitase 1 antibody 12406-1-APDownload protocol
IF protocol for Aconitase 1 antibody 12406-1-APDownload protocol
IP protocol for Aconitase 1 antibody 12406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomolecules

Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.

Authors - Sara Ceccacci
humanWB

Drug Des Devel Ther

Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation.

Authors - Chunguang Yang
human,mouseWB,IHC

Cell Biosci

Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease.

Authors - Li Niu