Aconitase 1 Polyclonal antibody

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  WB analysis using 12406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:5000) with HepG2 cells lysate 1925 ug.

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  IP experiment of HepG2 using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:5000) with HepG2 cells lysate 1925 ug.

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  IHC staining of human stomach using 12406-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12406-1-AP (Aconitase 1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 12406-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Aconitase 1 antibody (12406-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Aconitase 1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 12406-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, RIP, ELISA

Synonyms

ACO1, ACO 1, EC:4.2.1.3, Citrate hydro-lyase, Aconitase1

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• RIP: See 2 publications below

Product Information

12406-1-AP targets Aconitase 1 in WB, IHC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aconitase 1 fusion protein Ag3083
• Full Name: aconitase 1, soluble
• Calculated Molecular Weight: 889 aa, 98 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC018103
• Gene Symbol: Aconitase 1
• Gene ID (NCBI): 48
• RRID: AB_10642942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P21399
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	iScience; Asparagine starvation suppresses histone demethylation through iron depletion; Authors - Jie Jiang ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.; Authors - Haipeng Cheng
human,mouse	WB,IHC	Cell Biosci; Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease.; Authors - Li Niu
human	WB	Drug Des Devel Ther; Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation.; Authors - Chunguang Yang
human	WB	Biomolecules; Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.; Authors - Sara Ceccacci
human	WB	JOJ Ophthalmol; Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases.; Authors - Lalit Pukhrambam Singh