Aconitase 1 Polyclonal antibody

Aconitase 1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12406-1-AP

Synonyms

ACO1, Aconitase, Aconitase 1, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1

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Validation Data Gallery

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  WB analysis of mouse kidney using 12406-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12406-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12406-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12406-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:300) with mouse liver tissue lysate 2400 ug.

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  IF Staining of U2OS using 12406-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 12406-1-AP (Aconitase 1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HepG2 cells, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

12406-1-AP targets Aconitase 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, mosue
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aconitase 1 fusion protein Ag3083
• Full Name: aconitase 1, soluble
• Calculated molecular weight: 889 aa, 98 kDa
• Observed molecular weight: 98 kDa
• GenBank accession number: BC018103
• Gene symbol: ACO1
• Gene ID (NCBI): 48
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Biomolecules; Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.; Authors - Sara Ceccacci
human	WB	JOJ Ophthalmol; Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases.; Authors - Lalit Pukhrambam Singh
human	WB	Drug Des Devel Ther; Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation.; Authors - Chunguang Yang
human,mouse	WB,IHC	Cell Biosci; Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease.; Authors - Li Niu
mosue	WB	Biochim Biophys Acta Mol Basis Dis; Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.; Authors - Haipeng Cheng