Aconitase 1 Polyclonal antibody
Aconitase 1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 12406-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IF detected in||U2OS cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12406-1-AP targets Aconitase 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Aconitase 1 fusion protein Ag3083|
|Full Name||aconitase 1, soluble|
|Calculated molecular weight||889 aa, 98 kDa|
|Observed molecular weight||98 kDa|
|GenBank accession number||BC018103|
|Gene ID (NCBI)||48|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.
|Product Specific Protocols|
|WB protocol for Aconitase 1 antibody 12406-1-AP||Download protocol|
|IF protocol for Aconitase 1 antibody 12406-1-AP||Download protocol|
|IP protocol for Aconitase 1 antibody 12406-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases.
J Exp Clin Cancer Res
KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2