Aconitase 1 Polyclonal antibody

Aconitase 1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12406-1-AP


ACO1, Aconitase, Aconitase 1, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells, mouse liver tissue
Positive IF detected inU2OS cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12406-1-AP targets Aconitase 1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aconitase 1 fusion protein Ag3083
Full Name aconitase 1, soluble
Calculated Molecular Weight 889 aa, 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC018103
Gene Symbol ACO1
Gene ID (NCBI) 48
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.


Product Specific Protocols
WB protocol for Aconitase 1 antibody 12406-1-APDownload protocol
IF protocol for Aconitase 1 antibody 12406-1-APDownload protocol
IP protocol for Aconitase 1 antibody 12406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochim Biophys Acta Mol Basis Dis

Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.

Authors - Haipeng Cheng

Cell Biosci

Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease.

Authors - Li Niu

Drug Des Devel Ther

Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation.

Authors - Chunguang Yang


Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.

Authors - Sara Ceccacci

JOJ Ophthalmol

Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases.

Authors - Lalit Pukhrambam Singh

J Exp Clin Cancer Res

KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2

Authors - Hui Zhou