Aconitase 1 Polyclonal antibody
Aconitase 1 Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 12406-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, HepG2 cells, mouse liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IF detected in | U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
Product Information
12406-1-AP targets Aconitase 1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Aconitase 1 fusion protein Ag3083 |
Full Name | aconitase 1, soluble |
Calculated molecular weight | 889 aa, 98 kDa |
Observed molecular weight | 98 kDa |
GenBank accession number | BC018103 |
Gene symbol | ACO1 |
Gene ID (NCBI) | 48 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
IF protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
IP protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomolecules Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite. | ||
Drug Des Devel Ther Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation. | ||
Cell Biosci Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease. |