CoraLite® Plus 488-conjugated Aconitase 1 Polyclonal antibody

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  IF Staining of mouse liver using CL488-12406

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Aconitase 1 antibody (CL488-12406) at dilution of 1:200.

• 

  IF Staining of mouse liver using CL488-12406

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Aconitase 1 antibody (CL488-12406) at dilution of 1:200.

• Featured Product
• KD/KO Validated

Aconitase 1 Polyclonal Antibody for IF-P

Cat No. CL488-12406

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

ACO1, Aconitase, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1

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Tested Applications

• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12406 targets Aconitase 1 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3083

  Product name: Recombinant human ACO1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 540-890 aa of BC018103

  Sequence: GRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

• Full Name: aconitase 1, soluble
• Calculated Molecular Weight: 889 aa, 98 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC018103
• Gene Symbol: Aconitase 1
• Gene ID (NCBI): 48
• RRID: AB_3083926
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P21399
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.