ACOX1 Monoclonal antibody, PBS Only

ACOX1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E12E10

Cat no : 68017-1-PBS

Synonyms

ACOX, ACOX1, AOX, PALMCOX, Palmitoyl CoA oxidase, SCOX

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Validation Data Gallery

• 

  WB analysis using 68017-1-Ig (same clone as 68017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 68017-1-Ig (same clone as 68017-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68017-1-Ig (same clone as 68017-1-PBS)

  WB result of ACOX1 antibody (68017-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACOX1 transfected HepG2 cells. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68017-1-Ig (same clone as 68017-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68017-1-PBS targets ACOX1 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACOX1 fusion protein Ag28037
• Full Name: acyl-Coenzyme A oxidase 1, palmitoyl
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC010425
• Gene Symbol: ACOX1
• Gene ID (NCBI): 51
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).