ACOX1 Monoclonal antibody

ACOX1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3E12E10

Cat no : 68017-1-Ig

Synonyms

ACOX, ACOX1, AOX, PALMCOX, Palmitoyl CoA oxidase, SCOX



Tested Applications

Positive WB detected inLNCaP cells, pig liver tissue, rat liver tissue, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

68017-1-Ig targets ACOX1 in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACOX1 fusion protein Ag28037
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Calculated molecular weight 74 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC010425
Gene symbol ACOX1
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Protocols

Product Specific Protocols
WB protocol for ACOX1 antibody 68017-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Phytomedicine

An integrated network pharmacology and cell metabolomics approach to reveal the role of rhein, a novel PPARα agonist, against renal fibrosis by activating the PPARα-CPT1A axis.

Authors - Qiming Xiao
ratWB

Front Pharmacol

Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease

Authors - Xinhui Liu