|Positive WB detected in||LNCaP cells, pig liver tissue, rat liver tissue, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Sample-dependent, check data in validation data gallery|
68017-1-Ig targets ACOX1 in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
|Tested Reactivity||Human, Rat, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ACOX1 fusion protein Ag28037|
|Full Name||acyl-Coenzyme A oxidase 1, palmitoyl|
|Calculated molecular weight||74 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC010425|
|Gene ID (NCBI)||51|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).
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