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ADAM10 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 25900-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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ADAM10 Antibody IHC human lung cancer tissue 25900-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ADAM10 Antibody IHC human lung cancer tissue 25900-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ADAM10 Antibody WB A549 cells 25900-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 25900-1-AP( ADAM10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
A549 cells, NIH/3T3 cells, SW 1990 cells
Positive IHC detected in:
human lung cancer tissue, human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ADAM10 fusion protein Ag23078
Full name:
ADAM metallopeptidase domain 10
Calculated molecular weight:
748aa,84 kDa
Observed molecular weight:
70,90 kDa
GenBank accession number:
BC126253
Gene ID (NCBI):
102
Gene symbol
ADAM10
Synonyms
AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM

Background

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).


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