ADAM10 Polyclonal antibody
ADAM10 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 25900-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, NIH/3T3 cells, SW 1990 cells |
| Positive IHC detected in | human lung cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 25900-1-AP is ADAM10 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ADAM10 fusion protein Ag23078 |
| Full Name | ADAM metallopeptidase domain 10 |
| Calculated molecular weight | 748 aa, 84 kDa |
| Observed molecular weight | 70 kDa, 90 kDa |
| GenBank accession number | BC126253 |
| Gene symbol | ADAM10 |
| Gene ID (NCBI) | 102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM10 antibody 25900-1-AP | Download protocol |
| IHC protocol for ADAM10 antibody 25900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biosci Rep The cellular expression and proteolytic processing of the amyloid precursor protein is independent of TDP-43. | ||
Biomed Pharmacother Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122. | ||
Biochem Biophys Res Commun ADAM10 and ADAM17 are degraded by lysosomal pathway via asparagine endopeptidase. | ||
J Cancer MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer.
| ||
J Proteomics Bottom up proteomics identifies neuronal differentiation pathway networks activated by cathepsin inhibition treatment in neuroblastoma cells that are enhanced by concurrent 13-cis retinoic acid treatment. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH David (Verified Customer) (09-02-2019) | Used for immunoblot at 1:1000 which gives good, clear bands at the expected molecular weight, but does require long exposure times (30µg total protein, 10-15 minutes exposure). 1:500 would probably be a better dilution and this will be used for future experiments. It can be difficult to obtain good ADAM10 antibodies, but after trying several, this is the best.
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