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ADAM10 Polyclonal antibody
ADAM10 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (1)
WB, IHC, IF, ELISA
Cat no : 25900-1-AP
|Positive WB detected in||NIH/3T3 cells, mouse brain tissue, rat brain tissue|
|Positive IHC detected in||human lung cancer tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 15 publications below|
|IHC||See 2 publications below|
|IF||See 3 publications below|
25900-1-AP targets ADAM10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ADAM10 fusion protein Ag23078|
|Full Name||ADAM metallopeptidase domain 10|
|Calculated molecular weight||748 aa, 84 kDa|
|Observed molecular weight||60-70 kDa, 90 kDa|
|GenBank accession number||BC126253|
|Gene ID (NCBI)||102|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).
|Product Specific Protocols|
|WB protocol for ADAM10 antibody 25900-1-AP||Download protocol|
|IHC protocol for ADAM10 antibody 25900-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.
IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.
Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling.
Front Cell Dev Biol
NLGN3 Upregulates Expression of ADAM10 to Promote the Cleavage of NLGN3 via Activating the LYN Pathway in Human Gliomas.
Front Cell Dev Biol
Epigenetic Regulation of a Disintegrin and Metalloproteinase (ADAM) Transcription in Colorectal Cancer Cells: Involvement of β-Catenin, BRG1, and KDM4.
J Alzheimers Dis
Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimer's Disease.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
David (Verified Customer) (09-02-2019)
Used for immunoblot at 1:1000 which gives good, clear bands at the expected molecular weight, but does require long exposure times (30µg total protein, 10-15 minutes exposure). 1:500 would probably be a better dilution and this will be used for future experiments. It can be difficult to obtain good ADAM10 antibodies, but after trying several, this is the best.