ADAM10 Polyclonal antibody

• Featured Product
• KD/KO Validated

ADAM10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (2)

Cat no : 25900-1-AP

Synonyms

Mammalian disintegrin-metalloprotease, MADM, KUZ, EC:3.4.24.81, Disintegrin and metalloproteinase domain-containing protein 10

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Validation Data Gallery

• 

  WB analysis using 25900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25900-1-AP (ADAM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 25900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 25900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25900-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25900-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human lung cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

25900-1-AP targets ADAM10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM10 fusion protein Ag23078
• Full Name: ADAM metallopeptidase domain 10
• Calculated Molecular Weight: 748 aa, 84 kDa
• Observed Molecular Weight: 60-70 kDa, 90 kDa
• GenBank Accession Number: BC126253
• Gene Symbol: ADAM10
• Gene ID (NCBI): 102
• RRID: AB_2880291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).

Publications

Species	Application	Title
human,mouse	WB	Aging Cell; Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease; Authors - Guoxin Zhang
human	WB,IF	Cell Death Dis; Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.; Authors - Bo Wu
human,mouse	WB,IHC,IF	Pharmacol Res; IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.; Authors - Xin Fang
human	WB	Cell Prolif; Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling.; Authors - Yanmiao Huo
mouse	WB	J Neurosci; Novel Social Stimulation Ameliorates Memory Deficit in Alzheimer's Disease Model through Activating α-Secretase; Authors - Qiaoyun Ren
human	WB	Front Cell Dev Biol; NLGN3 Upregulates Expression of ADAM10 to Promote the Cleavage of NLGN3 via Activating the LYN Pathway in Human Gliomas.; Authors - Ning-Ning Dang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain

ADAM10 Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:25900-1-AP)

FH

David (Verified Customer) (09-02-2019)

Used for immunoblot at 1:1000 which gives good, clear bands at the expected molecular weight, but does require long exposure times (30µg total protein, 10-15 minutes exposure). 1:500 would probably be a better dilution and this will be used for future experiments. It can be difficult to obtain good ADAM10 antibodies, but after trying several, this is the best.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human neuroblastoma