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ADAM10 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 25900-1-AP

1 customer review

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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ADAM10 Antibody IHC human lung cancer tissue 25900-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ADAM10 Antibody IHC human lung cancer tissue 25900-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ADAM10 Antibody WB A549 cells 25900-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 25900-1-AP( ADAM10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
A549 cells, NIH/3T3 cells, SW 1990 cells
Positive IHC detected in:
human lung cancer tissue, human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ADAM10 fusion protein Ag23078
Full name:
ADAM metallopeptidase domain 10
Calculated molecular weight:
748aa,84 kDa
Observed molecular weight:
70,90 kDa
GenBank accession number:
BC126253
Gene ID (NCBI):
102
Gene symbol
ADAM10
Synonyms
AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM

Background

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ADAM10 Rabbit Polyclonal antibody

David (Verified Customer), 09-03-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
Human neuroblastoma
Lot Number
Used for immunoblot at 1:1000 which gives good, clear bands at the expected molecular weight, but does require long exposure times (30µg total protein, 10-15 minutes exposure). 1:500 would probably be a better dilution and this will be used for future experiments. It can be difficult to obtain good ADAM10 antibodies, but after trying several, this is the best.

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