ADAM17 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 84292-4-RR (same clone as 84292-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84292-4-RR (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01169-3

  Sandwich ELISA standard curve of MP01169-3, Human ADAM17 Recombinant Matched Antibody Pair - PBS only. 84292-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32418. 84292-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP01169-3

  The mean ADAM17 concentration was determined to be 18.51 ng/mL in THP-1 cell extract based on a 4.4 mg/mL extract load and 13.76 ng/mL in A549 cell extract based on a 2.7 mg/mL extract load.

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  Sample test of MP01169-3

  Serum of six individual healthy human donors was measured. The human ADAM17 concentration of detected samples was determined to be 1,898.9 pg/mL with a range of 21.5 - 8,096.2 ng/mL.

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  IHC staining of human rectal cancer using 84292-4-RR (same clone as 84292-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84292-4-RR (ADAM17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

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  FC experiment of HCT 116 using 84292-4-RR (same clone as 84292-4-PBS)

  1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug ADAM17 Recombinant antibody (84292-4-RR, Clone:241305A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84292-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84292-4-RR against Human ADAM17 were performed. The affinity constant is 0.385 nM.

ADAM17 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84292-4-PBS

Clone No.241305A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86

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Product Information

84292-4-PBS targets ADAM17 as part of a matched antibody pair:

MP01169-3: 84292-3-PBS capture and 84292-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADAM17 fusion protein Ag32418
• Full Name: ADAM metallopeptidase domain 17
• Calculated Molecular Weight: 824 aa, 93 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC136783
• Gene Symbol: ADAM17
• Gene ID (NCBI): 6868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P78536
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).