ADAMTS4 Polyclonal antibody

ADAMTS4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11865-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

ADAM TS 4, ADAM TS4, ADAMTS 2, ADAMTS 4, ADAMTS4, ADMP 1, Aggrecanase 1, KIAA0688

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11865-1-AP targets ADAMTS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAMTS4 fusion protein Ag2443 Predict reactive species
Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 4
Calculated Molecular Weight 837 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC030812
Gene Symbol ADAMTS4
Gene ID (NCBI) 9507
RRIDAB_2877799
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75173
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).

Protocols

Product Specific Protocols
WB protocol for ADAMTS4 antibody 11865-1-APDownload protocol
IHC protocol for ADAMTS4 antibody 11865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Adv Sci (Weinh)

Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis

Authors - Yizhen Huang
mouseWB

Adv Sci (Weinh)

Inhibition of Heat Shock Protein 90β by Catalpol: A Potential Therapeutic Approach for Alleviating Inflammation-Induced Cartilage Injuries in Osteoarthritis

Authors - Zhenwei Zhou
ratWB

Acta Biomater

ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration

Authors - Tianhui Zhang
pigWB,IHC

Bone Joint Res

Suramin attenuates intervertebral disc degeneration by inhibiting NF-κB signalling pathway.

Authors - Zi-Miao Liu
mouseWB

Mol Med Rep

Acetyl zingerone ameliorates osteoarthritis by inhibiting chondrocyte programmed cell death

Authors - Xu Chen
humanWB

Inflammation

Simvastatin Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation by Suppressing the NF-kB and MAPK Pathways in Nucleus Pulposus Cells.

Authors - Ji Tu
Loading...