ADAMTS4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82744-2-RR (same clone as 82744-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82744-2-RR (ADAMTS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82744-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82744-2-RR (same clone as 82744-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ADAMTS4 Recombinant antibody (82744-2-RR, Clone:4F9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82744-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82744-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82744-2-RR against Human ADAMTS4 were performed. The affinity constant is 0.632 nM.

ADAMTS4 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 82744-2-PBS

Clone No.4F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

ADAM-TS 4, ADAMTS 4, ADAM TS4, ADAM TS 4, A disintegrin and metalloproteinase with thrombospondin motifs 4

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Product Information

82744-2-PBS targets ADAMTS4 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2443

  Product name: Recombinant human ADAMTS4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2-339 aa of BC030812

  Sequence: SQTGSHPGRGLAGRWLWGAQPCLLLPIVPLSWLVWLLLLLLASLLPSARLASPLPREEEIVFPEKLNGSVLPGSGAPARLLCRLQAFGETLLLELEQDSGVQVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALLGVLQYRGAELHLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPLGSPSPRPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSLVVTRLVILGSGEEGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTRQVRPQSAPQAMHCTILRSATT

• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 4
• Calculated Molecular Weight: 837 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC030812
• Gene Symbol: ADAMTS4
• Gene ID (NCBI): 9507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75173
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).