ADAMTS4 Recombinant antibody

ADAMTS4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4F9

Cat no : 82744-2-RR

Synonyms

ADAM-TS 4, ADAMTS 4, ADAM TS4, ADAM TS 4, A disintegrin and metalloproteinase with thrombospondin motifs 4



Tested Applications

Positive WB detected inTHP-1 cells, HEK-293T cells, SH-SY5Y cells, Caco-2 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82744-2-RR targets ADAMTS4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ADAMTS4 fusion protein Ag2443
Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 4
Calculated Molecular Weight 837 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC030812
Gene Symbol ADAMTS4
Gene ID (NCBI) 9507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).

Protocols

Product Specific Protocols
WB protocol for ADAMTS4 antibody 82744-2-RRDownload protocol
Standard Protocols
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