AHCY Polyclonal antibody

• 

  WB analysis using 10757-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10757-2-AP

  WB result of AHCY antibody (10757-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHCY transfected HeLa cells.

• 

  WB analysis of mouse liver using 10757-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10757-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10757-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10757-2-AP (AHCY antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

AHCY Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10757-2-AP

Publications(28)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

SAHH, EC:3.13.2.1

---

Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells, mouse liver tissue, HeLa cells, Jurkat cells, rat liver tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

10757-2-AP targets AHCY in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, branchiostoma belcheri
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1208

  Product name: Recombinant human AHCY protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 116-432 aa of BC011606

  Sequence: QTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY

• Full Name: S-adenosylhomocysteine hydrolase
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC011606
• Gene Symbol: AHCY
• Gene ID (NCBI): 191
• RRID: AB_2289488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23526
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh
human	WB, IP	Nat Commun; H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.; Authors - Jichun Zhou ; KD Validated
human	WB	Autophagy; AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner.; Authors - Wei Huang ; KD Validated
human	WB	Oncogene; PMID: 27775072; Authors - T Zhong
human	WB,IHC	Clin Cancer Res; Digital transcript profile analysis with aRNA-LongSAGE validates FERMT1 as a potential novel prognostic marker for colon cancer.; Authors - Fan Junwei J
mouse	WB	Arterioscler Thromb Vasc Biol; Increased plasma S-adenosylhomocysteine-accelerated atherosclerosis is associated with epigenetic regulation of endoplasmic reticulum stress in apoE-/- mice.; Authors - Yunjun Xiao ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kamal (Verified Customer) (01-26-2024)

Antibody worked well for western blot analysis when diluted with 1X TBST at the provided dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse liver tissue