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  • KD/KO Validated

AHR Monoclonal antibody

AHR Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b


Human, mouse, rat and More (1)







Cat no : 67785-1-Ig


Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HEK-293 cells, HSC-T6 cells, 4T1 cells, PC-3 cells, HeLa cells, Caco-2 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, mouse testis tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67785-1-Ig targets AHR in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AHR fusion protein Ag28935
Full Name aryl hydrocarbon receptor
Calculated Molecular Weight 848 aa, 96 kDa
Observed Molecular Weight105-110 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)


Product Specific Protocols
WB protocol for AHR antibody 67785-1-IgDownload protocol
IHC protocol for AHR antibody 67785-1-IgDownload protocol
IF protocol for AHR antibody 67785-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Phytother Res

Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity

Authors - Xiaojing Wang

Ecotoxicol Environ Saf

Hesperetin protects hippocampal neurons from the neurotoxicity of Aflatoxin B1 in mice

Authors - Chao Song

Biomater Sci

Glioma-targeted multifunctional nanoparticles to co-deliver camptothecin and curcumin for enhanced chemo-immunotherapy.

Authors - Zian Wang

J Inflamm Res

MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis

Authors - Yunzhi Liu


Ginsenoside Rb1 prevents osteoporosis via the AHR/PRELP/NF-κB signaling axis.

Authors - Dan Zhang
  • KD Validated

Food Funct

Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.

Authors - Chengcheng Yang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Bartosz (Verified Customer) (02-09-2023)

Perfect Abs, the bands are clear and very strong, even in case of small of amount of protein, loaded into gel (20 ug). Just perfect, highly recommend.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: SH-SY5Y cells; A549 cells; CACO-2 cells; BJ cells