AKAP3 Polyclonal antibody

AKAP3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13907-1-AP

Synonyms

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13907-1-AP targets AKAP3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP3 fusion protein Ag4885
Full Name A kinase (PRKA) anchor protein 3
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC047535
Gene Symbol AKAP3
Gene ID (NCBI) 10566
RRIDAB_2273887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

Protocols

Product Specific Protocols
WB protocol for AKAP3 antibody 13907-1-APDownload protocol
IHC protocol for AKAP3 antibody 13907-1-APDownload protocol
IF protocol for AKAP3 antibody 13907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.

Authors - Tong Liu
mouseWB

Proc Natl Acad Sci U S A

SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

Authors - Haruhiko Miyata
mouseIF

Development

The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development

Authors - Anja Beckers
mouseWB,IF

J Genet Genomics

Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

Authors - Yaling Wang
mouseIF

Development

The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development.

Authors - Anja Beckers
pigWB

Front Cell Dev Biol

Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.

Authors - Yongjie Xu