AKAP3 Polyclonal antibody

AKAP3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13907-1-AP

Synonyms

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13907-1-AP targets AKAP3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP3 fusion protein Ag4885
Full Name A kinase (PRKA) anchor protein 3
Calculated molecular weight 95 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC047535
Gene symbol AKAP3
Gene ID (NCBI) 10566
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

Protocols

Product Specific Protocols
WB protocol for AKAP3 antibody 13907-1-APDownload protocol
IHC protocol for AKAP3 antibody 13907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Clin Genet

Biallelic mutations in PMFBP1 cause acephalic spermatozoa.

Authors - Yan-Wei Sha
humanCoIP

Nat Commun

Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.

Authors - Ying Shen
mouseIF

Dev Biol

CFAP43 modulates ciliary beating in mouse and Xenopus.

Authors - Ev Rachev
mouseWB, IF, IHC, IP

Sci Rep

Interactome of the negative regulator of nuclear import BRCA1-binding protein 2.

Authors - Shadma Fatima
mouseWB

J Cell Sci

CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.

Authors - Samantha A M Young
mouseWB

Chemosphere

Fluoride exposure alters the ultra-structure of sperm flagellum via reducing key protein expressions in testis.

Authors - Chen Liang