AKAP4 Polyclonal antibody

AKAP4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24986-1-AP

Synonyms

A kinase anchor protein 4, A kinase anchor protein 82 kDa, AKAP 4, AKAP 82, AKAP4, AKAP82, CT99, FSC1, hAKAP82, HI, p82, PRKA4



Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24986-1-AP is AKAP4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP4 fusion protein Ag21762
Full Name A kinase (PRKA) anchor protein 4
Calculated molecular weight 854 aa, 94 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC126252
Gene symbol AKAP4
Gene ID (NCBI) 8852
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP4, also named as A-kinase anchor protein 82 kDa, is a 854 amino acid protein, which belongs to the AKAP110 family. AKAP4 localizes to the principle piece of the sperm flagellum and is only expressed in round spermatids. AKAP4 is a major structural component of sperm fibrous sheath and plays a role in sperm motility.

Protocols

Product Specific Protocols
WB protocol for AKAP4 antibody 24986-1-APDownload protocol
IHC protocol for AKAP4 antibody 24986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.

Authors - Tong Liu