ALPL Monoclonal antibody

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  WB analysis using 68719-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68719-1-Ig (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat adrenal gland using 68719-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68719-1-Ig (ALPL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ALPL Monoclonal Antibody for WB, IHC, ELISA

Cat No. 68719-1-Ig

Clone No.2E3C1

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rabbit

Applications

WB, IHC, ELISA

Synonyms

Alkaline phosphatase, 2E3C1, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme

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Tested Applications

• Positive WB detected in: pig kidney tissue, rabbit kidney tissue
• Positive IHC detected in: rat adrenal gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68719-1-Ig targets ALPL in WB, IHC, ELISA applications and shows reactivity with human, pig, rabbit samples.

• Tested Reactivity: human, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALPL fusion protein Ag33678
• Full Name: alkaline phosphatase, liver/bone/kidney
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC021289
• Gene Symbol: ALPL
• Gene ID (NCBI): 249
• RRID: AB_3670412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05186
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).