ALPL Monoclonal antibody, PBS Only

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  WB analysis using 68719-1-Ig (same clone as 68719-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68719-1-Ig (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68719-1-PBS in a different storage buffer formulation.

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  IHC staining of rat adrenal gland using 68719-1-Ig (same clone as 68719-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68719-1-Ig (ALPL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68719-1-PBS in a different storage buffer formulation.

ALPL Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 68719-1-PBS

Clone No.2E3C1

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

Alkaline phosphatase, 2E3C1, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme

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Product Information

68719-1-PBS targets ALPL in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rabbit samples.

• Tested Reactivity: human, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALPL fusion protein Ag33678
• Full Name: alkaline phosphatase, liver/bone/kidney
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC021289
• Gene Symbol: ALPL
• Gene ID (NCBI): 249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05186
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).