AMOTL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

AMOTL2 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (2)

Cat no : 23351-1-AP

Synonyms

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 23351-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23351-1-AP (AMOTL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below

Product Information

The immunogen of 23351-1-AP is AMOTL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMOTL2 fusion protein Ag19953
• Full Name: angiomotin like 2
• Calculated molecular weight: 779 aa, 86 kDa
• Observed molecular weight: 100 kDa
• GenBank accession number: BC011454
• Gene symbol: AMOTL2
• Gene ID (NCBI): 51421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Hypoxic Tumor-Derived Exosomal Long Noncoding RNA UCA1 Promotes Angiogenesis via miR-96-5p/AMOTL2 in Pancreatic Cancer.; Authors - Zengya Guo ; KD Validated
human	WB	Mol Ther Nucleic Acids; Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO.; Authors - Xue Zhang
human	WB	Arthritis Res Ther; Regulation of type I interferon signature by VGLL3 in the fibroblast-like synoviocytes of rheumatoid arthritis patients via targeting the Hippo pathway; Authors - Yu Du
human	WB	Sci Rep; MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis.; Authors - Diala Kantar ; KD Validated
human	WB	Mol Biol Cell; AMOT130 drives BMP-SMAD signaling at the apical membrane in polarized cells.; Authors - Patrizia Brunner ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Florian (Verified Customer) (10-13-2021)

10% SDS-PAGE 1st AB incubation ON

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human breast cancer cell line (HCC1806)

AMOTL2 Antibody Western Blot validation (1:500 dilution) in Human breast cancer cell line (HCC1806) (Cat no:23351-1-AP)

FH

Lisa (Verified Customer) (07-08-2020)

Two specific bands (60 and 100 kDa) and two others non specific.This antibody is working by WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1/600
• Cell Tissue Type: MCF7 cell line

AMOTL2 Antibody Western Blot validation (1/600 dilution) in MCF7 cell line (Cat no:23351-1-AP)