ANGPTL3 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 86193-1-RR (same clone as 86193-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86193-1-RR (ANGPTL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86193-1-PBS in a different storage buffer formulation.

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  Sample test of MP02294-1

  The mean ANGPTL3 concentration was determined to be 14.8 ng/mL in human serum.

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  Cytometric bead array standard curve of MP02294-1

  Cytometric bead array standard curve of MP02294-1, ANGPTL3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86193-2-PBS. Detection antibody: 86193-1-PBS. Standard: Ag2573. Range: 1.563-200 ng/mL.

ANGPTL3 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86193-1-PBS

Clone No.250808F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

ANG-5, Angiopoietin like protein 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3

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Product Information

86193-1-PBS targets ANGPTL3 as part of a matched antibody pair:

MP02294-1: 86193-2-PBS capture and 86193-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ANGPTL3 fusion protein Ag2573
• Full Name: angiopoietin-like 3
• Calculated Molecular Weight: 460 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC058287
• Gene Symbol: ANGPTL3
• Gene ID (NCBI): 27329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y5C1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.