ANGPTL7 Polyclonal antibody

• Featured Product
• KD/KO Validated

ANGPTL7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 10396-1-AP

Synonyms

Angiopoietin-like protein 7, Angiopoietin-like factor, Angiopoietin related protein 7, Angiopoietin like protein 7, Angiopoietin like factor

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Validation Data Gallery

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  WB analysis using 10396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10396-1-AP (ANGPTL7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10396-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10396-1-AP (ANGPTL7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 10396-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ANGPTL7 antibody (10396-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10396-1-AP targets ANGPTL7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANGPTL7 fusion protein Ag0596
• Full Name: angiopoietin-like 7
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC001881
• Gene Symbol: ANGPTL7
• Gene ID (NCBI): 10218
• RRID: AB_2057556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANGPTL7, also named as CDT6, is a member of the angiopoietin-like (ANGPTL) family of proteins, which are important regulators of Angiogenesis. It is a secreted 45-kDa glycoprotein that folds into a homotetramer structure linked by disulfide bonds. ANGPTL7 was originally discovered in the stromal layer of the corne, and moderately expressed in the human trabecular meshwork. The chromosomal location of the ANGPTL7 gene (1p36.22) is within the GLC3B locus (1p36.2-1p36.1) of primary congenital glaucoma, supporting ANGPTL7 as a candidate glaucoma gene. ANGPTL7 could have a pathogenic role in glaucoma, and may serve as a potential therapeutic target.

Publications

Species	Application	Title
human	WB	FASEB J; Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation.; Authors - Tong Xu ; KD Validated
mouse	IF	Osteoarthritis Cartilage; Effects of mechanical stress and deficiency of dihydrotestosterone or 17β-estradiol on Temporomandibular Joint Osteoarthritis in mice.; Authors - T Ootake
human	IHC	Front Mol Biosci; An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.; Authors - Hongpan Zhang
human	WB,IF	Genes Cells; Evidence for a role of angiopoietin-like 7 (ANGPTL7) in extracellular matrix formation of the human trabecular meshwork: implications for glaucoma.; Authors - Comes Núria N
human	WB	Commun Biol; ANGPTL7, a therapeutic target for increased intraocular pressure and glaucoma; Authors - Kavita Praveen
human	WB	Hum Mol Genet; Rare protective variants and glaucoma-relevant cell stressors modulate angiopoietin-like 7 expression; Authors - Inas F Aboobakar

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (01-05-2024)

The antibody worked well for immunofluorescence on frozen mouse eye sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Eye