ANKRD2 Antibody

ANKRD2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11821-1-AP

Synonyms

ANKRD2, ARPP, hArpp



Tested Applications

Positive WB detected inhuman heart tissue, mouse skeletal muscle tissue, human skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman kidney tissue, human lung tissue, human skeletal muscle tissue, human testis tissue, human brain tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11821-1-AP targets ANKRD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD2 fusion protein Ag2389
Full Name ankyrin repeat domain 2 (stretch responsive muscle)
Calculated molecular weight 37 aa, 2 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC020817
Gene symbol ANKRD2
Gene ID (NCBI) 26287
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with Ankrd1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. Ankrd2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus

Protocols

Product Specific Protocols
WB protocol for ANKRD2 antibody 11821-1-APDownload protocol
IHC protocol for ANKRD2 antibody 11821-1-APDownload protocol
IF protocol for ANKRD2 antibody 11821-1-APDownload protocol
IP protocol for ANKRD2 antibody 11821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Appl Physiol

Early depression of Ankrd2 and Csrp3 mRNAs in the polyribosomal and whole tissue fractions in skeletal muscle with decreased voluntary running.

Authors - Roberts Michael D MD
mouseWB

Mol Biol Cell

Ankrd2/ARPP is a novel Akt2 specific substrate and regulates myogenic differentiation upon cellular exposure to H(2)O(2).

Authors - Cenni Vittoria V
mouseIF

Cell Death Dis

The histone code reader Spin1 controls skeletal muscle development.

Authors - Holger Greschik